Bst DNA Polymerase (Exonuclease Minus)

Bst DNA Polymerase (Exonuclease Minus)

Ready for LAMP (Loop-mediated Isothermal Amplification)

Product Overview:

Large Fragment of Bst DNA Polymerase, Exonuclease Minus, is isolated from a Bacillus stearothermophilus. It catalyzes 5’ 3’ synthesis of DNA and lacks 5’ 3’ and 3’ 5’ exonuclease activities.

Source:  A recombinant E. coli strain carrying the Bst DNA Polymerase gene (Large fragment).

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Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Concentration Price
P0045 2,000 U 8 U/µL 109 €
P0046 10,000 U 8 U/µL 398 €

Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • High Purity: greater than 99% confirmed by SDS PAGE.
  • Reverse transcription activity.
  • Optimized: Increased activity.
  • Strong strand displacement activity.
  • Risk-free: product covered by our Quality 100% Guarantee.

Specifications

Unit definition:
One unit catalyzes the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 65 C in 20 mMTris-HCl pH 8.8, 10 mM (NH4)2SO4,10 mM KCl, 2 mM MgSO4, 0.1% Triton X-100, 30 nM M13mp18 ssDNA, 70 nMM13 sequencing primer(-47) 24-mer 200 µM dGTP, dATP, dTTP, dCTP (a mix of unlabeled and [33P]dCTP) and 0.1 mg/mL BSA.

Includes

Includes for 2,000 U:
– Bst DNA Polymerase (8 U/μL)
– Reaction Buffer (10x)
– 100mM MgSO4 solution

Download documentation

Data Sheet
Brochure

Applications

  • Nucleic acid amplification methods.
  • Whole genome amplification.
  • Multiple displacement amplification.
  • DNA Sequencing.

 

Some uses for this product may require licenses. Canvax does not encourage or support the unauthorized or unlicensed use of patented nucleic acid amplification processes for isothermal amplification, whole genome amplification (WGA), multiple displacement amplification (MDA), and Next Generation sequencing. It is the sole responsibility of the buyer to ensure that use of the product does not infringe the patent rights of third parties.

Quality Control

  • Purity: >99% by SDS PAGE.
  • Exempt of nucleases (endo-, exo and ribonucleases) activities guaranteed by appropriate quality tests.
  • 10 µL of the enzyme was tested for E. coli genomic DNA contamination by PCR amplifying with the E.coli 16S ribosomal primers.

Storage, Shipping & Guarantee​

  • Shipped in: Gel pack.
  • Storage: -20 °C (NON Frost-Free Freezer).
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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Frequently asked questions

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Customers Review

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