FastCONTROL™ p2V-SEAP/ LUC-III – Dual Reporter

FastCONTROL™ p2V-SEAP/ LUC-III – Dual Reporter

For a Faster, Convenient & Flexible Target Cell Transfection Control

Product Overview:

FastCONTROL™ Dual Reporters are fast, convenient and flexible target cell transfection control ideal for high co-expression of two reporter genes drive for ubiquitous, strong and constitutive promoters. FastCONTROL™ p2V-SEAP/ LUC-III Dual Reporter vector contains cytomegalovirus promoter (Pcmv) to drive the expression of both secreted embryonic alkaline phosphatase (SEAP) and firefly luciferase from Photinus pyralis (LUC).

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Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
 PC0119 15 µg (100 assays) 237 €
PC0119-Plus 15 µg + 0.2 mL (100 assays) 298 €

Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Fast: available combination of 2 cell location markers in one vector.
  • Convenient: available in Lentiviral, Retroviral or non-viral vectors, for immediate in vivo and in vitro expression.
  • Compatible with transient or stable transfections.
  • Flexible: available with different mammalian resistance markers and Dual Reporter genes combination for different cell location.
  • Risk-free: product covered by our Quality 100% Guarantee.

Includes

Includes for 100 assays:

– 15 µL FastCONTROL™ Dual Reporter (1 µg/µL)

 

Plus Version – Includes for 100 assays:

– 15 µL FastCONTROL™ Dual Reporter (1 µg/µL)

– 0.2 mL CANFAST™ Transfection Reagent (1 mg/mL)

Download documentation

Data Sheet
Brochure

Applications

  • Control for assessing the efficiency of transfection in mammalian cells.
  • Targering of different cell locations.
  • Obtention of cell lines with reporter proteins.

Tables, Figures & Protocols

p2V-SEAP -  LUC-III

Quality Control

  • The quantity and quality of purified DNA attend to:

– Ratio 260/ 280 (1.8 – 2.0).
– Agarose gel Electrophoresis.
– Digestion with Restriction Endonucleases.

  • Transient Transfection CHO-K1 (625 cells / well with these vectors) provides a SEAP activity >1,000 fold higher than untransfected cells themselves.
  • The surface expression of NGFR in transiently transfected CHO > 60%.
  • Expression of eGFP in transient intracellular CHO > 60%.

Storage, Shipping & Guarantee​

  • Shipped in: Gel pack.
  • Storage: FastCONTROL™ Dual Reporter Plasmid (-20 ºC) and CANFAST™ (4 ºC).
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Current Promotions


Citations Reward Program, get 50€ Amazon gift card Learn more Here


Lab Start-Up Program for new labs/companies Learn more Here


Bulk & Volume discounts for larger quantities Learn more Here

Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

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