FDG – Galactosidase Assay Kit (Fluorimetric)

FDG – Galactosidase Assay Kit (Fluorimetric)

Efficient, Easy & Highly Sensitive tool, Fluorimetric based, to quickly measure levels of active β-galactosidase

Product Overview:

FDG β-Galactosidase Assay Kit is an efficient, easy and highly sensitive tool to quickly measure the levels of active β-galactosidase expressed in cells transfected with plasmids expressing Lac Z.

Lac Z is often used reporter gene in experiments transfection because the β-galactosidase is very resistant to proteolytic degradation and its activity is easily measured. β-galactosidase performs the hydrolysis of fluorescein di-β-D galactopyranoside (FDG) into a fluorescein that can be detected at excitation/emission = 490/525 nm. The concentration of β-galactosidase is proportional to fluorescence produced.measure levels of active β-galactosidase expressed in cells transfected with plasmids expressing Lac Z.

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Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
CA083 500 assays 259 €  219 €

Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Fast and convenient. 
  • Easy-to-use method to quantify the enzyme expression in transfected cells.
  • Sensitive: measure ß-galactosidase at femtogram level.
  • Risk-free: product covered by our Quality 100% Guarantee.

Includes

Includes for 500 assays:

 

– 55 mL Reaction Buffer
– 25 mL Lysis Buffer
– 25 mL Stop solution
– 20 μL β-galactosidase enzyme (0.1 mg/mL)
– 500 μL β-Mercaptoethanol
– 125 μL FDG solution (10 mM)

Download documentation

Data Sheet

Applications

  • Measurement of β-Galactosidase activity in the lysates of transfected cell.

Tables, Figures & Protocols

fdg

Quality Control

  • Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.

Storage, Shipping & Guarantee​

  • Shipped in: Gel Pack.
  • Storage: Upon receipt, store the kit at -20 ºC. β-galactosidase substrate must be protected from light.
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

 

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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Frequently asked questions

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Customers Review

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