Bile Acid Receptor

Bile Acid Receptor

ExpressMAX™ Expression-ready Human GPCR ORF Clones

Product Overview:

Bile Acid GPCR (GPBAR1) functions as a cell surface Receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway and internalization of the Receptor. The Receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in three transcript variants encoding the same protein.

Quote request

Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
G0512-A 15 µL (15 assays) 148 €
G0512-B 15 µL (15 assays) 897 €
G0512-Plus 15 µL + 0.2 mL (15 assays) 958 €

Press here to learn the difference between this three products.

 

Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Native structure.
  • Wide range: a large collection of ExpressMAX™ GPCR ORF Clones availables.
  • Highest levels of GPCR expression: on surface cell lines, > 50% of TAG detection by cytometry.
  • Wide spectrum: of strong constitutive promoters.
  • Complete solution: it contains all necessary elements for maximum receptor expression.
  • Ready-to-use solution: avoids cloning steps, DNA ready to transfect.
  • Easy protocol and detection: the whole procedure is simple, with minimal handling.
  • Neomycin resistance.
  • Ampicillin bacterial selection.

Specifications

  • Plasmid  size: 6332 bp
  • Promoter: pMLV
  • ORF Sequence (GenBank based): NM_170699
  • Protein Sequence (SwissProt): Q8TDU6
  • Bacterial Selection Antibiotics:  Ampicillin
  • Mammalian Selection Antibiotic: Neomycin
  • Alternative names: BG37, TGR5, M-BAR or GPCR19

Includes

Includes for 15 μg:

ExpressMAX™ GPCR ORF Clones (Cat. No. -A & B)
– 15 µg ExpressMAX™ Mammalian Expression Vector (1 mg/mL)

 

Mammalian Transfection Kits (Cat. No. -PLUS):
– 15 µg ExpressMAX™ Mammalian Expression Vector (1 mg/mL)
– 0.2 mL CANFAST™ Transfection Reagent

(15 µg = 15 assays)

Download documentation

Data Sheet
Brochure

Applications

  • Functional assays, as protein immobilization, cellular localization and other functional assays.
  • High-throughput and large scale protein production and purification.
  • Reverse transfection arrays and nucleic acid programmable protein arrays (NAPPA).

Quality Control

The quantity and quality of purified DNA is checked by:

  • Ratio 260/ 280.
  • Agarose gel electrophoresis.
  • Digestion with restriction endonucleases.

Storage, Shipping & Guarantee​

  • Shipped in: Dry Ice.
  • Storage: ExpressMAX™ GPCR ORF Clones (-20 ºC) and CANFAST™ (4 ºC).
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Current Promotions


Citations Reward Program, get 50€ Amazon gift card Learn more Here


Lab Start-Up Program for new labs/companies Learn more Here


Bulk & Volume discounts for larger quantities Learn more Here

Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

  • slidebg1

    Related products

    Ampicillin Sodium Salt

    Ampicillin is a penicillin-like antibiotic effective against gram-positive, gram-negative and anaerobic bacteria. Differs from penicillin only by the presence of an amino group. Like penicillin, ampicillin is susceptible to β-lactamase, which hydrolyzes the β-lactam ring. It is widely used in cell...


    Learn more
  • slidebg1

    Related products

    CANFAST™ Transfection Reagent

    CANFAST™ is a Highly Efficient, Ready-to-& Non-Toxic new generation cationic Polymer. It has important features as DNA condensation and endosomal release, which improves gene transfection...


    Learn more
  • slidebg1

    Related products

    pOnebyOne™ I – Neo Bicistronic Mammalian Expression Kit

    pOnebyOne™ are extremely efficient, accurate and flexible Bicistronic Mammalian Expression Kits that contains an expression cassette based in 2A sequence Breakthrough technology. The expression cassette of pOnebyOne™ I (with Neomycin resistance) incorporates the cytomegalovirus early promoter that precedes 2A sequence in frame with...


    Learn more