Anaphylatoxin GPR77

Anaphylatoxin GPR77

ExpressMAX™ Expression-ready Human GPCR ORF Clones

Product Overview:

GPR77, is a Receptor for the chemotactic and inflammatory peptide anaphylatoxins C5a, C4a and C3a and their desarginated derivatives. It is expressed on various immune cells and non-immune cells such as adipocytes, astrocytes, fibroblasts, immature dendritic cells, macrophages, mast cells, monocytes, neurons, neutrophils. The Receptor binds complement factor C5a with high affinity.

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Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
G0508-A 15 µL (15 assays) 148 €
G0508-B 15 µL (15 assays) 897 €
G0508-Plus 15 µL + 0.2 mL (15 assays) 958 €

Press here to learn the difference between this three products.

 

Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Native structure.
  • Wide range: a large collection of ExpressMAX™ GPCR ORF Clones availables.
  • Highest levels of GPCR expression: on surface cell lines, > 50% of TAG detection by cytometry.
  • Wide spectrum: of strong constitutive promoters.
  • Complete solution: it contains all necessary elements for maximum receptor expression.
  • Ready-to-use solution: avoids cloning steps, DNA ready to transfect.
  • Easy protocol and detection: the whole procedure is simple, with minimal handling.
  • Neomycin resistance.
  • Ampicillin bacterial selection.

Specifications

  • Plasmid  size: 6353 bp
  • Promoter: pMLV
  • ORF Sequence (GenBank based): NM_018485
  • Protein Sequence (SwissProt): Q9P296
  • Bacterial Selection Antibiotics:  Ampicillin
  • Mammalian Selection Antibiotic: Neomycin
  • Alternative names: C5L2, GPR77 or C5AR2

Includes

Includes for 15 μg:

ExpressMAX™ GPCR ORF Clones (Cat. No. -A & B)
– 15 µg ExpressMAX™ Mammalian Expression Vector (1 mg/mL)

 

Mammalian Transfection Kits (Cat. No. -PLUS):
– 15 µg ExpressMAX™ Mammalian Expression Vector (1 mg/mL)
– 0.2 mL CANFAST™ Transfection Reagent

(15 µg = 15 assays)

Download documentation

Data Sheet
Brochure

Applications

  • Functional assays, as protein immobilization, cellular localization and other functional assays.
  • High-throughput and large scale protein production and purification.
  • Reverse transfection arrays and nucleic acid programmable protein arrays (NAPPA).

Quality Control

The quantity and quality of purified DNA is checked by:

  • Ratio 260/ 280.
  • Agarose gel electrophoresis.
  • Digestion with restriction endonucleases.

Storage, Shipping & Guarantee​

  • Shipped in: Dry Ice.
  • Storage: ExpressMAX™ GPCR ORF Clones (-20 ºC) and CANFAST™ (4 ºC).
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

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