Horse-Power™ Taq DNA Polymerase MasterMix (2x)

Horse-Power™ Taq DNA Polymerase MasterMix (2x)

Highly Purified (>98%) for Routine Amplifications in Ready-to-use format

Product Overview:

Horse-Power™ Taq DNA Polymerase MasterMix (2x) is an optimized ready-to-use MasterMix that contains all PCR reaction components: dNTPs, PCR buffer, Mg2+ and Taq DNA polymerase. Only primers and template need to be added. The convenient MasterMix (2x) formulation saves time and eliminates the risk of contamination due to a reduced number of pipetting steps required.

Horse-Power™ Taq DNA Polymerase is a versatile and thermostable recombinant enzyme produced in an E. coli strain, which carries the cloned pol gene from Thermus aquaticus. The enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading).

Quote request

Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
P0035  2 x 1.25 mL (250 rxn) 84 €

Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Highest purity: greater than 98% confirmed by SDS-PAGE.
  • Highest quality: high activity, specificity, thermostability and performance in PCR.
  • Highly efficient: reactivation buffer improved.
  • Thermostable: half-life at 94 ºC is 40 minutes.
  • Adds extra nucleotides: preferentially adenine, without template at 3´ends leaving 3´overhangs PCR fragments.
  • Incorporates modified nucleotides: biotinylated, fluorescently labelled, etc.
  • Molecular Weight: 94 kDa.
  • Convenient: available in different concentrations, sizes and solutions.
  • Risk-free: product covered by our Quality 100% Guarantee.

Specifications

Assay conditions:

25 mM Tris-HCl pH 9.0 at 25 °C, 50 mM KCl, 2 mM MgCl2, 0.1 mg/mL gelatine, 200 µM dATP, dGTP, dTTP, 100 µM [α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA.

 

Unit definition:

One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.

Includes

Includes for 1.25 mL:

– 2 x 1.25 mL Horse-Power™ Taq DNA polymerase MasterMix (2x) (2 U/μL)

Download documentation

Data Sheet
Brochure

Applications

  • Routine amplifications.
  • Colony screening (see Horse-Power™ Red-Taq DNA Polymerase).
  • Amplifications up to 5 kb using plasmid, viral or genomic DNA as template.
  • PCR fragments amplification for TA or GC cloning.

Tables, Figures & Protocols

Amplification Taq DNA Polymerase

Quality Control

  • Functionally tested in PCR.
  • Free of bacterial DNA (by qPCR).
  • Exempt of nucleases (endo-, exo and ribonucleases) activities guaranteed by appropriate quality tests.

Storage, Shipping & Guarantee​

  • Shipped in: Gel pack.
  • Storage: -20 °C (NON Frost-Free Freezer).
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Citations

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Current Promotions


Citations Reward Program, get 50€ Amazon gift card Learn more Here


Lab Start-Up Program for new labs/companies Learn more Here


Bulk & Volume discounts for larger quantities Learn more Here

Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

  • slidebg1

    Related products

    Horse-Power™ Taq DNA Polymerase MasterMix (2x)

    Horse-Power™ Taq DNA Polymerase MasterMix (2x) is an optimized ready-to-use MasterMix that contains all PCR reaction components: dNTPs, PCR buffer, Mg2+ and Taq DNA polymerase. Only primers and template need to be added. The convenient MasterMix (2x) formulation saves time and eliminates the risk of contamination...


    Learn more
  • slidebg1

    Related products

    Horse-Power™ Taq DNA Polymerase with TruePure™ dNTPs

    Horse-Power™ Taq DNA Polymerase is a pure, versatile and thermostable recombinant enzyme produced in an E. coli strain, which carries the cloned pol gene from Thermus aquaticus. The enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but...


    Learn more
  • slidebg1

    Related products

    pSpark® TA DNA Cloning Kit

    pSpark® TA is efficient, stable and easy-to-use DNA cloning vector based on an optimized TA technology for cloning single 3 ́-adenine overhanging DNA. The vectors are prepared by digestion of pSpark® TA at EcoRV site and the subsequent addition of...


    Learn more
  • slidebg1

    Related products

    BrightMAX™ 25 – 700 bp DNA Ladder

    BrightMAX™ 25 – 700 bp DNA Ladder  is a precise and highly stable DNA Marker suitable for sizing double-stranded DNA from 25 bp to 700 bp. Ready-to-use, it contains loading dyes with one or two migration visualisation dyes. It is composed...


    Learn more
  • slidebg1

    Related products

    TBE Buffer (10x) (pH 8.3)

    TBE is a high quality, reliable and convenient buffer for DNA and RNA polyacrilamide gel electrophoresis. It is useful due its basic pH, which allows migrations of DNA through the gel toward the positive anode. TBE buffers are used for...


    Learn more