Horse-Power™ Taq DNA Polymerase

Horse-Power™ Taq DNA Polymerase

Highly Purified (>98%) for Routine Amplifications

Product Overview:

Horse-Power™ Taq DNA Polymerase is a pure, versatile and thermostable recombinant enzyme produced in an E. coli strain, which carries the cloned pol gene from Thermus aquaticus. The enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading).

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Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Concentration Price
P0023-S 200 U 5 U/μL 32 €
P0023 500 U 5 U/μL 59 €  39€
P0024 1,000 U 5 U/μL 117 €
P0025 500 U 1 U/μL 63 €
P0019 5,000 U 1 U/μL 309 €
P0020 10,000 U 5 U/μL 496 €

Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM Partnerships.

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Highest purity: greater than 98% confirmed by SDS-PAGE.
  • Highest quality: high activity, specificity, thermostability and performance in PCR.
  • Highly efficient: reactivation buffer improved.
  • Thermostable: half-life at 94 ºC is 40 minutes.
  • Adds extra nucleotides: preferentially adenine, without template at 3´ends leaving 3´overhangs PCR fragments.
  • Incorporates modified nucleotides: biotinylated, fluorescently labelled, etc.
  • Molecular Weight: 94 kDa.
  • Convenient: available in different concentrations, sizes and solutions.
  • Complete solution: includes MgCl2.
  • Risk-free: product covered by our Quality 100% Guarantee.

Specifications

Assay conditions:

25 mM Tris-HCl pH 9.0 at 25 °C, 50 mM KCl, 2 mM MgCl2, 0.1 mg/mL gelatine, 200 µM dATP, dGTP, dTTP, 100 µM [α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA.

 

Unit definition:

One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.

Includes

Concentration: 5 U/μL – Includes for 500 U:
– 100 μL Horse-Power™ Taq Polymerase (5 U/μL)
– 25 mM MgCl2 (1.5 mL)
– 1.5 mL Buffer (10x)

 

Concentration: 1 U/μL – Includes for 500 U:

– 500 μL Horse-Power™ Taq Polymerase (1 U/μL)
– 25 mM MgCl2 (1.5 mL)
– 1.5 mL Buffer (10x)

Download documentation

Data Sheet
Brochure

Applications

  • Routine amplifications.
  • Colony screening (see Horse-Power™ Red-Taq DNA Polymerase).
  • Amplifications up to 5 kb using plasmid, viral or genomic DNA as template.
  • PCR fragments amplification for TA or GC cloning (preferably use a proofreading polymerase for cloning purposes combined with an efficient blunt cloning vector) (see pSpark® DNA Cloning System).

Tables, Figures & Protocols

Amplification Taq DNA Polymerase

Quality Control

  • Functionally tested in PCR.
  • Undetected bacterial DNA (by PCR).
  • Undetectable nucleases (endo-, exo, and ribonucleases) activities guaranteed by appropriate quality tests.

Storage, Shipping & Guarantee​

  • Shipped in: Gel pack.
  • Storage: -20 ºC (NON Frost-Free Freezer).
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Citations

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Current Promotions


Citations Reward Program, get 50€ Amazon gift card Learn more Here


Lab Start-Up Program for new labs/companies Learn more Here


Bulk & Volume discounts for larger quantities Learn more Here

Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

2 Responses

  • Eric Henderson dice:

    Better performance than other products double prices. Includes MgCl for free!

  • Manuel Pérez dice:

    Productos de la BBB, jeje, Buenos, Bonitos y Baratos y fabricados en España

  • Leave a Reply

    Productos de la BBB, jeje, Buenos, Bonitos y Baratos y fabricados en España

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