M-MLV Reverse Transcriptase

M-MLV Reverse Transcriptase

Encoded Moloney Murine Leukemia Virus, purified from E.coli (200 U/µL)

Product Overview:

M-MLV Reverse Transcriptase (MMLV-RT), encoded by Moloney Murine Leukemia Virus (MMLV) is an RNAdependent DNA polymerase that synthesizes the cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. MMLV-RT will also extend primers hybridized to single-stranded DNA.

Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.

Source: purified from E.coli strain harboring a plasmid that directs the synthesis of modified form of MMLV-RT.

MW: 69 KDa.

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Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
P0073 10,000 units 83 €  75 €
P0074 50,000 units 278 €

Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Concentration: 10 u/µL.
  • Full Solution: includes Reaction Buffer 5x.
  • Risk-free: product covered by our Quality 100% Guarantee.

Specifications

Unit definition:

One unit of the enzyme incorporates 1 nmol dTTP into acid-precipitable material in 10 minutes at 37 ° C, using poly(A) oligo dT as a template primer.

Includes

Includes:

– M-MLV Reverse Transcriptase (200 U/μL)

– Reaction Buffer 5x

Download documentation

Data Sheet
Brochure

Applications

  • RT PCR.
  • Synthesis of cDNA.
  • mRNA 5’-end Mapping by Primer Extension Analysis.
  • End-labeling of DNA.
  • Dideoxynucleotide Sequencing.

Quality Control

  • Exempt of nucleases (endo, exo and ribonucleases) activities.
  • Purity: >90% as judged by SDS-polyacrylamide gels with blue staining.

Storage, Shipping & Guarantee​

  • Shipped in: Gel pack.
  • Storage: -20 ºC (NON Frost-Free Freezer).
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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Frequently asked questions

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