M-MLV Reverse Transcriptase
Encoded Moloney Murine Leukemia Virus, purified from E.coli (200 U/µL)
M-MLV Reverse Transcriptase (MMLV-RT), encoded by Moloney Murine Leukemia Virus (MMLV) is an RNAdependent DNA polymerase that synthesizes the cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. MMLV-RT will also extend primers hybridized to single-stranded DNA.
Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.
Source: purified from E.coli strain harboring a plasmid that directs the synthesis of modified form of MMLV-RT.
MW: 69 KDa.Quote request
Availability: In stock! Ready-to-ship immediately
Catalog Numbers, Sizes & Prices
|P0074||50,000 units||278 €|
Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.
Advantages & Features
- Concentration: 10 u/µL.
- Full Solution: includes Reaction Buffer 5x.
- Risk-free: product covered by our Quality 100% Guarantee.
One unit of the enzyme incorporates 1 nmol dTTP into acid-precipitable material in 10 minutes at 37 ° C, using poly(A) oligo dT as a template primer.
– M-MLV Reverse Transcriptase (200 U/μL)
– Reaction Buffer 5x
- RT PCR.
- Synthesis of cDNA.
- mRNA 5’-end Mapping by Primer Extension Analysis.
- End-labeling of DNA.
- Dideoxynucleotide Sequencing.
- Exempt of nucleases (endo, exo and ribonucleases) activities.
- Purity: >90% as judged by SDS-polyacrylamide gels with blue staining.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
- Shelf Life: 24 months from the date of purchase, if it is properly stored.
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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