pMBL™ T-Vector DNA Cloning Kit

pMBL™ T-Vector DNA Cloning Kit

For a Efficient, Convenient & Fast Cloning of DNA fragments with A overhangs

Product Overview:

pMBL™ T-Vector DNA Cloning Kit is an efficient, convenient and fast system for the cloning of PCR products. The vector is prepared by cutting pMBL™ T-Vector with EcoRV and adding a 3´ terminal thymidine to both ends. These single 3´-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmids by preventing recircularization of the vector and providing a compatible overhang for PCR products generated by certain thermostable polymerases such as Horse-Power™ Taq DNA Polymerase.

These Polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments.

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Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
C0030 20 rxn 181 €

Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Highly efficient: greater than 90% white colonies in a transformation with supplied insert control.
  • Proven performance: more than 1,000 recombinant colonies expected in optimal conditions.
  • Fast and easy protocol: results from 15 min protocol.
  • Optimized: improve efficiency of ligation of a PCR product into the plasmid.
  • Compatible: overhang for ligation of PCR products preventing recircularization of the vector.
  • Designed by cutting the vector with EcoRV and adding a 3´ terminal thymidine to both ends.
  • Risk-free: product covered by our Quality 100% Guarantee.

Includes

Includes for 20 rxn:

– 20 µL pMBL™ T-Vector (50 ng/µL)

– 20 µL T4 DNA Ligase (5U/Weiss)

– 100 µL T4 DNA Ligase Buffer (10x)

– 5 µL Insert Control 600 bp (30 ng/µL)

Download documentation

Manual
Brochure

Applications

  • Cloning of PCR fragments into DNA Cloning vector.
  • Blue/white screening for recombinants.

Tables, Figures & Protocols

pMBL-T Vector

Quality Control

  • Functional test using a 600 pb PCR fragment.

Storage, Shipping & Guarantee​

  • Shipped in: Gel Pack.
  • Storage: -20 ºC (NON Frost-Free Freezer).
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Citations

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

One Response

  • Harry Payne dice:

    We used since more than 10 years because its great performance and easy use.

  • Leave a Reply

    We used since more than 10 years because its great performance and easy use.

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