pSpark® Done DNA Cloning Kit

pSpark® Done DNA Cloning Kit

For highly Efficient, Accurate & Easy Cloning of PCR fragments with EcoRI and NotI flanking the insertion site

Product Overview:

pSpark® Done is a highly efficient, accurate and easy-to-use DNA cloning system designed for cloning of blunt ended DNA with very high efficiency. The MCS of the pSpark® Done vector incorporates sequences on either side of the insert that are recognized by the restriction enzymes NotI and EcoRI. This allows the insert DNA to be removed with a single restriction digest using either of these enzymes

Quote request

Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
C0006-S 10 rxn 99 €
C0006 20 rxn 189 €

Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Optimized: recognition sites for Not I and EcoR I at either side for the insertion of the cloning point.
  • Flexible: allows removing the desired insert DNA with other restriction digestion.
  • Unprecedented efficiency: more than 2,500 positive colonies expected under optimal conditions.
  • Easy-to-use: eliminate screening of recombinants due to its its minumum background (lower than 1%).
  • Time-saving protocol: avoids any step required after PCR, just 19 minutes from PCR to plating.
  • Powerful: obtain 5-fold more positive colonies using 10-fold less DNA insert.
  • High stability: eliminates cloning bias or pitfalls.
  • Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers.
  • Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert.
  • Eliminates positive selection vector.
  • Cost avoidance: removes expensive primer phosphorylation use.
  • Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation.
  • Risk-free: product covered by our Quality 100% Guarantee.

Includes

Includes for 20 rxn:

– 20 µL pSpark® Done (20 ng/µL)

– 20 µL T4 DNA Ligase (5U/Weiss)

– 200 µL T4 DNA Ligase Buffer (5x)

– 150 µL PEG 6000 10x

– 5 µL Insert Control 1 kb (20 ng/µL)

Download documentation

Manual
MSDS
Brochure
White Paper

Applications

  • Cloning of High Fidelity PCR amplified products.
  • Production of ssDNA.
  • Blue/white screening for recombinants.
  • In vitro transcription from T7/SP6 dual-opposed promoters.
  • One restriction enzyme allows gene fragment excision.

Tables, Figures & Protocols

pSpark TA Done

Quality Control

  • Functional test using a 1.0 kb PCR fragment.

Advice

  • Recommendations: All pSpark® DNA cloning vectors are stable for at least 1 month at 4 °C and even at 20 – 25 °C for up to 2 days although storage temperatures above -20 °C are not recommended. In case of incident, such as a power failure, stored vectors should be tested with the supplied control insert before considering discarding it. However, please note that T4 DNA Ligase is extremely temperature-sensitive and storage temperatures above    -20 °C inactivates the enzyme.

Storage, Shipping & Guarantee​

  • Shipped in: Gel Pack.
  • Storage: -20 ºC (NON Frost-Free Freezer).
  • Shelf Life: 24 months from the date of purchase, if it is properly stored

Citations

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Current Promotions


Citations Reward Program, get 50€ Amazon gift card Learn more Here


Lab Start-Up Program for new labs/companies Learn more Here


Bulk & Volume discounts for larger quantities Learn more Here

Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

  • slidebg1

    Related products

    FastPANGEA™ Long PCR DNA Polymerase

    FastPANGEA™ Long PCR DNA Polymerase is an accurate and robust enzyme that combines Horse-Power™ Taq DNA Polymerase and a DNA proofreading Polymerase with 3’ to 5’ exonuclease activity that is optimized for PCR amplification of very long DNA templates (long...


    Learn more
  • slidebg1

    Related products

    FastPANGEA™ High Fidelity DNA Polymerase

    FastPANGEA™ High Fidelity DNA Polymerase is a second generation High-fidelity DNA Polymerase that offers extreme performance for all PCR applications. It generates long templates with an accuracy and speed previously unattainable with other thermostable DNA polymerases. The error rate of...


    Learn more
  • slidebg1

    Related products

    CVX5α™ Chemically Competent Cells

    CVX5α™ Chemically Competent Cells is a versatile, convenient and cost-effective solution for routine subcloning procedures or any application where the starting DNA is not limiting. It is calcium chloride-treated to facilitate attachment of the plasmid DNA to the competent cell membrane....


    Learn more
  • slidebg1

    Related products

    Ampicillin Sodium Salt

    Ampicillin is a penicillin-like antibiotic effective against gram-positive, gram-negative and anaerobic bacteria. Differs from penicillin only by the presence of an amino group. Like penicillin, ampicillin is susceptible to β-lactamase, which hydrolyzes the β-lactam ring. It is widely used in cell...


    Learn more