WideUSE™ Plasmid Midiprep Kit

WideUSE™ Plasmid Midiprep Kit

For a Fast, Simple & Highly Efficient routinary Isolation of High Quality Plasmid preparations in Midiprep format

Product Overview:

WideUSE™ Plasmid Midiprep Kit offers a fast, simple and highly efficient method for the routinary isolation of high quality Plasmid preparations in Midiprep format.

It introduces the Lysis control reagent (LCR) a color indicator which provides visual identification of optimum buffer mixing. This prevents common handling errors that lead to inefficient cell lysis and incomplete precipitation of SDS, genomic DNA and cell debris. This makes ideal for use by researchers who have not much experience with plasmid preparation as well as experienced scientists who want to be assured of maximum product yield.

Quote request

Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
AN0168 (Midiprep) 25 rxn 238 €  198 €

Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • High quality: of Plasmid DNA obtained, ready-to-use in all Molecular Biology applications. Achieve transfection grade plasmid purity.
  • Fast: results in few minutes.
  • Safe: avoids phenol-chloroform extraction.
  • Complete solution: includes specialized filters to optional remove cellular debris from lysates.
  • Midiprep format.
  • Technology: based on familiar anion-exchange columns.
  • Risk-free: product covered by our Quality 100% Guarantee.

Includes

Includes for Midiprep format:

– 110 mL Resuspension Solution

– 150 mL Lysis Solution (BLY)

– 110 mL Neutralization Solution

– 130 mL Equilibration Buffer

– 360 mL Wash Buffer (WB1)

– 220 mL Elution Buffer (EB)

– 1,500 µL Lysis control reagent (LCR)

– 25 mL TE Buffer

– 25 Plasmid Midi / Maxi Columns

– 200 µL RNase (50 mg/mL)

– 25 Folder Filters

 

Download documentation

Data Sheet

Applications

All Molecular Biology applications, such as:

  • Digestion with restriction enzymes.
  • Automated sequencing.
  • PCR template.
  • Bacterial transformation.
  • Transfection.

Quality Control

– Tested for the isolation of any plasmid DNA from transformed E.coli.

– The quality of purified DNA is analysed by:

  • Ratio 260/ 280.
  • Agarose gel electrophoresis.
  • Digestion with restriction endonucleases.

Storage, Shipping & Guarantee​

  • Shipped in: Ambient temperature.
  • Storage: All components should be stored at room temperature (15–25 °C) for up to 12 months without any reduction in performance. Store the RNAse A at -20 ºC. After addition of RNase A to Resuspension Solution can be stored at 4 ºC. Verify that the Lysis Solution (BLY) does not contain precipitated SDS due to the low temperatures. If necessary, dissolve the SDS heating at 37 °C.
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Current Promotions


Bulk & Volume discounts for larger quantities Learn more Here


Citations Reward Program, get 50€ Amazon gift card Learn more Here


Lab Start-Up Program for new labs/companies Learn more Here

Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

  • slidebg1

    Related products

    RNase A

    RNase A (Ribonuclease A) is a bovine pancreatic endoribonuclease that cleaves single-stranded RNA. It catalyzes the cleavage of the phosphodiester bond between the 5’-ribose of a nucleotide and the phosphate group attached to the 3’-ribose of an adjacent pyrimidine nucleotide....


    Learn more
  • slidebg1

    Related products

    WideUSE™ Plasmid Maxiprep Kit

    WideUSE™ Plasmid Maxiprep Kit offers a fast, simple and Highly efficient method for the routinary isolation of high quality Plasmid preparations in Maxiprep format. It introduces the Lysis control reagent (LCR) a color indicator which provides visual identification of optimum...


    Learn more
  • slidebg1

    Related products

    WideUSE™ Plasmid Purification Kit

    WideUse™ Plasmid Purification Kit offers a reliable, convenient and rapid method for routinary isolation of high quality plasmid preparations in mini format. The kit uses HigherPurity™ breakthrough technology based in DNA ability to bind silica in the presence of high...


    Learn more