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PCR Cloning methods (Life Science Blog)

Date: 2017-03-14 by Canvax
PCR Cloning methods (Life Science Blog)

In the last 20 years, applications for the polymerase chain reaction (PCR) technique (Saiki et al., 1988) have been developed in almost every field in Molecular Biology. However, in a large number of situations, observing an amplification band on a gel is not enough. Sequencing, directed mutagenesis, polymorphic transcription (widely used in drug-genomics identification), obtaining […]

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DNA Cloning Introduction II (Life Science Blog)

Date: 2017-03-14 by Canvax
DNA Cloning Introduction II (Life Science Blog)

Cloning of products obtained by PCR is a process widely used in many laboratories. Depending on the DNA Polymerase used in the amplification, we obtain fragments with a base but without the corresponding template at the 3′ end, or blunt fragments.   Several methodologies have been developed to clone both types of fragments. Within these […]

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DNA Cloning Introduction (Life Science Blog)

Date: 2017-02-22 by Canvax
DNA Cloning Introduction (Life Science Blog)

What is DNA cloning? By definition, the general term “cloning” means obtaining multiples copies of something, let it be individuals of an animal species, or molecules. DNA cloning is a Molecular Biology technique that allows us to get multiple copies of identical sequence-specific double-stranded DNA fragments, for various purposes.     How is DNA cloning […]

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