In the last 20 years, applications for the polymerase chain reaction (PCR) technique (Saiki et al., 1988) have been developed in almost every field in Molecular Biology. However, in a large number of situations, observing an amplification band on a gel is not enough. Sequencing, directed mutagenesis, polymorphic transcription (widely used in drug-genomics identification), obtaining […]
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Cloning of products obtained by PCR is a process widely used in many laboratories. Depending on the DNA Polymerase used in the amplification, we obtain fragments with a base but without the corresponding template at the 3′ end, or blunt fragments. Several methodologies have been developed to clone both types of fragments. Within these […]
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What is DNA cloning? By definition, the general term “cloning” means obtaining multiples copies of something, let it be individuals of an animal species, or molecules. DNA cloning is a Molecular Biology technique that allows us to get multiple copies of identical sequence-specific double-stranded DNA fragments, for various purposes. How is DNA cloning […]
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