TEV Protease

TEV Protease

Product Overview:

TEV Protease is a highly sequence-specific cysteine protease from Tobacco Etch Virus (TEV). It is a member of the PA clan of chymotrypsin-like proteases. Due to its high sequence specificity, it is frequently used for the controlled cleavage of fusion proteins in vitro and in vivo. The recognition sequence of the enzyme is Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) and […]

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mRNA Cap 2-O-Methyltransferase

mRNA Cap 2-O-Methyltransferase

Product Overview:

Recombinant mRNA Cap 2′-O-Methyltransferase from Vaccinia virus is produced in Escherichia coli. It catalyzes the addition of a methyl group at the 2′-O position of the first nucleotide adjacent to the cap structure at the 5′ end of the RNA. The enzyme utilizes SAM as a methyl donor to methylate capped RNA (Cap 0) resulting […]

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Vaccinia Capping System

Vaccinia Capping System

Product Overview:

Vaccinia Capping System is a kit that provides the required components to catalyze the addition of 7- methylguanylate cap structures (Cap 0) to the 5′ end of RNA. Vaccinia Capping Enzyme (VCE) is a heterodimeric protein produced in Escherichia coli.   GMP-grade is also available. Learn more

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Poly(A) Polymerase

Poly(A) Polymerase

Product Overview:

Poly(A) Polymerase I from Escherichia coli, is a very active recombinant enzyme produced by a overproducer strain of E.coli. The enzyme adds a long tail of adenine nucleotides at the 3’ end of messenger RNA using ATP as donor.   GMP-grade is also available. Learn more.

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CVX™ Poly(A) Tailing Kit

CVX™ Poly(A) Tailing Kit

Product Overview:

The Poly(A) Polymerase enzyme adds a chain of adenine nucleotides to the RNA. This process, called polyadenylation, increases the stability of RNA in eukaryotic cells and prevents its degradation. The CVX™ Poly(A) Tailing kit is designed to add a poly(A) tail to the 3’ end of (m)RNA transcripts generated by in vitro transcription with CVX™ […]

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T7 Fluorescein RNA Labeling Kit

T7 Fluorescein RNA Labeling Kit

Product Overview:

T7 Fluorescein RNA Labeling Kit is designed to generate randomly fluorescein-modified RNA probes by in vitro transcription. Such probes are suited for in situ hybridization and Northern blot hybridization experiments. The fluorescein labeled analog of uridine triphosphate (Fluorescein-12-UTP) is used for labeling RNA probes. Fluorescein-12-UTP is efficiently incorporated into RNA as substitute for its natural […]

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CVX™ T7 ARCA mRNA Synthesis Kit

CVX™ T7 ARCA mRNA Synthesis Kit

Product Overview:

CVX™ T7 ARCA mRNA Synthesis Kit is designed for in vitro ARCA-capped (m)RNA Synthesis by T7 RNA Polymerase. The cap structure is added to the mRNA by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA). ARCA is incorporated into mRNA exclusively in the correct orientation, generating capped mRNA that is more efficiently translated. By using a […]

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CVX™ SP6 Transcription Kit

CVX™ SP6 Transcription Kit

Product Overview:

CVX™ SP6 Transcription Kit is designed for in vitro RNA synthesis by SP6 RNA Polymerase from linearized plasmid DNA templates or DNA templates generated by PCR. Synthesized RNA can subsequently be used for in vitro translation, RNase protection assays, RNA splicing, hybridization assays and Northern Blot experiments. The kit contains sufficient reagents for 50 reactions […]

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Lithium Chloride Precipitation Solution

Lithium Chloride Precipitation Solution

Product Overview:

Lithium Chloride Precipitation Solution is formulated 7.5 M LiCl, 50 mM EDTA colorless solutions for RNA isolation and in vitro transcription. A final concentration of 2.5M LiCl is effective in precipitation of RNA larger than 300 nucleotides without the addition of ethanol, this method of precipitation selectively precipitates RNA and does not efficiently precipitate DNA, […]

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T7 Digoxigenin RNA Labeling Kit

T7 Digoxigenin RNA Labeling Kit

Product Overview:

T7 Digoxigenin RNA Labeling Kit is designed to generate randomly Digoxigenin-modified RNA probes by in vitrotranscription with Dioxigenin-11-UTP. Digoxigenin (DIG) is a steroid hapten derived from plants of the genus Digitalis (D. lanata, and the purple foxglove D. purpurea). It is widely used for generating non-radioactive probes and is an alternative to biotinylation. Like biotin, […]

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