Adrenergic ADRB2
ExpressMAX™ Expression-ready Human GPCR ORF Clones
Product Overview:
Beta-2-adrenergic Receptor is a member of beta adrenergic Receptors, which mediate catecholamine-induced activation of adenylate cyclase through the action of G proteins. It is located primarily in the CNS, heart, kidney and muscle where it is involved in smooth muscle relaxation (e.g. bronchodilation). ADRB2 is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. Different polymorphic forms, point mutations and/or downregulation of the gene are associated with nocturnal asthma, obesity and type 2 diabetes.
Quote requestAvailability: In stock! Ready-to-ship immediately
Catalog Numbers, Sizes & Prices
| Cat. No. | Size | Price |
| G0505-A | 15 µL (12-well plate) | 148 € |
| G0505-B | Bacterial Stock | 298 € |
| G0505-Plus | 15 µL (12-well plate) + 0.2 mL | 688 € |
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Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.
Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.
Advantages & Features
- Native structure.
- Wide range: a large collection of ExpressMAX™ GPCR ORF Clones availables.
- Highest levels of GPCR expression: on surface cell lines, > 50% of TAG detection by cytometry.
- Wide spectrum: of strong constitutive promoters.
- Complete solution: it contains all necessary elements for maximum receptor expression.
- Ready-to-use solution: avoids cloning steps, DNA ready to transfect.
- Easy protocol and detection: the whole procedure is simple, with minimal handling.
- Neomycin resistance.
- Ampicillin bacterial selection.
Specifications
- Plasmid size: 7290 bp
- Promoter: pEF1a
- ORF Sequence (GenBank based): NM_000024.5
- Protein Sequence (SwissProt): P07550
- Bacterial Selection Antibiotics: Ampicillin
- Mammalian Selection Antibiotic: Neomycin
- Alternative names: BAR, B2AR, ADRBR or ADRB2R
Includes
Includes:
ExpressMAX™ GPCR ORF Clones (Cat. No. -A)
– 15 µg ExpressMAX™ Mammalian Expression Vector (1 mg/mL).
ExpressMAX™ GPCR ORF Clones (Cat. No. – B)
– Vector as transformed Bacteria in stab culture or a Glycerol Stock format.
Mammalian Transfection Kits (Cat. No. – PLUS):
– 15 µg ExpressMAX™ Mammalian Expression Vector (1 mg/mL).
– 0.2 mL CANFAST™ Transfection Reagent.
Download documentation
Data SheetBrochureApplications
- Functional assays, as protein immobilization, cellular localization and other functional assays.
- High-throughput and large scale protein production and purification.
- Reverse transfection arrays and nucleic acid programmable protein arrays (NAPPA).
Quality Control
The quantity and quality of purified DNAis checked by:
- Ratio 260/ 280.
- Agarose gel electrophoresis.
- Digestion with restriction endonucleases.
Storage, Shipping & Guarantee
- Shipped in: Dry Ice.
- Storage: ExpressMAX™ GPCR ORF Clones (-20 ºC) and CANFAST™ (4 ºC).
- Shelf Life: 24 months from the date of purchase, if it is properly stored.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
