BL21 (DE3) Competent Cells
Chemically Competent Escherichia coli Cells optimized for Protein Expression of T7 RNA Polymerase-based systems
Product Overview:
BL21 (DE3) Competent Cells are Chemically Competent Escherichia coli Cells used for Protein Expression of T7 RNA Polymerase-based Cystems. These cells are resistant to the lytic bacteriophages T1 and T5. The BL21 (DE3) strain is a derivative of E. coli B. It is deficient in both lon and ompT proteases resulting in superior isolation of intact Recombinant Proteins. The host is a lysogen of DE3 and, therefore, carries a chromosomal copy of the T7 RNA polymerase gene that is controlled by the lacUV5 promoter. The strain utilizes the T7 RNA promoter to control protein expression. IPTG is used to induce Expression of the T7 RNA Polymerase.
Genotype: F¯ ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 *lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
Quote requestAvailability: In stock! Ready-to-ship immediately
Catalog Numbers, Sizes & Prices
| Cat. No. | Size | Price |
| C0035 | 20 rxn | 197 € |
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Advantages & Features
- High-level of Protein Expression.
- Transformation Efficiency: ≥ 106 cfu/μg.
- Deficient in proteases Ion and OmpT.
Specifications
Genotype: F¯ ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 *lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
Includes
Includes for 20 rxn:
– 10 µL BL21 (DE3) Competent Cells Control Plasmid (0.1 ng/µL)
– 10 x 200μL BL21 (DE3) Competent Cells
– Dry Ice
Download documentation
Data SheetApplications
- Protein Expression using the T7 Expression System.
Quality Control
- BL21(DE3) Competent Cells consistently yield > 1.0 × 106 colony-forming units/µg competent cells control plasmid when transformed with non-saturating amounts of DNA (0.2ng/100 µL cells).
Storage, Shipping & Guarantee
- Shipment with Dry Ice.
- Storage: Upon receipt of the kit, immediately store the components at -80 °C. Storage at -20 °C will result in a significant decrease in transformation efficiency. Please note that Competent Cells are very sensitive to cycles of freezing and thawing and should not be exposed to temperature variations.
- Shelf Life: 6 months from the date of purchase, if it is properly stored.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
