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• Extremely fast and easy procedure: it takes 5 minutes to results with minimal handling steps.
• Reproducible yields: higher than 80% DNA Recovery (0.7 – 1% agarose) and reproducible yields of pure DNA.
• Versatile: compatible with a wide spectrum of size fragments, suitable since 100 bp up or any kind of agarose and gel buffer systems.
• Pure genomic DNA: ready-to-use in all Molecular Biology applications.
• Risk-free: product covered by our Quality 100% Guarantee.

Includes for 50 rxn:

– 50 CleanEasy™ MiniSpin Columns

– 50 Collection tubes (2 mL)

– 60 mL QG Buffer

– 11.25 mL PE Buffer

– 10 mL EB Buffer

– Purification of DNA fragments (obtained by PCR or digestion with restriction enzymes) from agarose gels.
– Purified DNA suitable for all common Molecular Biology applications, such as:

• PCR.
• Cloning.
• DNA sequencing.
• Southern blot analysis.

                Protocol:

• Tested in the purification of a 0.5 kb DNA fragment excised from 2% agarose gel.
• The purified band is analysed in agarose gel electrophoresis and quantify by spectrophotometry Ratio 260/ 280 (1.8-2).

• Shipped in: Ambient Temperature.
• Storage: All components can be stored at Room Temperature (15–25 °C).
• Guaranteed: All components are stable for 2 years, if stored properly.

• Romero, I., Casillas-Gonzalez, A. C., Carrazana-Villalba, S. J., Escribano, M. I., Merodio, C., & Sanchez-Ballesta, M. T. (2018). Impact of high CO2 levels on heat shock proteins during postharvest storage of table grapes at low temperature. Functional in vitro characterization of VVIHSP18. 1. Postharvest Biology and Technology, 145, 108-116.
• Moreno, B., Vivas, A., Nogales, R., Macci, C., Masciandaro, G., & Benitez, E. (2009). Restoring biochemical activity and bacterial diversity in a trichloroethylene-contaminated soil: the reclamation effect of vermicomposted olive wastes. Environmental Science and Pollution Research, 16(3), 253-264.
• Vivas, A., Moreno, B., Garcia-Rodriguez, S., & Benítez, E. (2009). Assessing the impact of composting and vermicomposting on bacterial community size and structure, and microbial functional diversity of an olive-mill waste. Bioresource Technology, 100(3), 1319-1326.
• Moreno, B., Nogales, R., Macci, C., Masciandaro, G., & Benitez, E. (2011). Microbial eco-physiological profiles to estimate the biological restoration of a trichloroethylene-contaminated soil. Ecological Indicators, 11(6), 1563-1571.
• Moreno, B., Cañizares, R., Nuñez, R., & Benitez, E. (2013). Genetic diversity of bacterial β-glucosidase-encoding genes as a function of soil management. Biology and fertility of soils, 49(6), 735-745.
• Rubio, L. A., Peinado, M. J., Echávarri, A., Ruiz, R., Suárez-Pereira, E., Mellet, C. O., & Fernández, J. M. G. (2014). A Di-D-Fructose Dianhydride-Enriched Caramel Modulates Pig Fecal Microbiota Composition. Advances in Microbiology, 2014.
• Cañizares, R., Moreno, B., & Benitez, E. (2012). Biochemical characterization with detection and expression of bacterial β-glucosidase encoding genes of a Mediterranean soil under different long-term management practices. Biology and Fertility of Soils, 48(6), 651-663.
• Cañizares, R., Benitez, E., & Ogunseitan, O. A. (2011). Molecular analyses of β-glucosidase diversity and function in soil. European Journal of Soil Biology, 47(1), 1-8.
• Vivas, A., Moreno, B., Garcia-Rodriguez, S., & Benítez, E. (2009). Assessing the impact of composting and vermicomposting on bacterial community size and structure, and microbial functional diversity of an olive-mill waste. Bioresource Technology, 100(3), 1319-1326.
• Sánchez-Castro, I., Amador-García, A., Moreno-Romero, C., López-Fernández, M., Phrommavanh, V., Nos, J., … & Merroun, M. L. (2017). Screening of bacterial strains isolated from uranium mill tailings porewaters for bioremediation purposes. Journal of environmental radioactivity, 166, 130-141.
• Peinado, M. J., Ruiz, R., Echávarri, A., & Rubio, L. A. (2012). Garlic derivative propyl propane thiosulfonate is effective against broiler enteropathogens in vivo. Poultry science, 91(9), 2148-2157.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.