DNase I

DNase I

High Quality & Proven Performance in RNA In Vitro Transcription Reactions

Product Overview:

DNase I is a recombinant endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, to release di-, tri- and oligonucleotide products (on average producing tetranucleotides) with 5’-phosphorylated and 3’-hydroxylated ends.

It acts on single-stranded DNA, double-stranded DNA, RNA-DNA hybrids and chromatin. DNase I requires bivalent cations (Mg2⁺ and Ca2⁺) for maximal activity.

GMP-grade reagent also available. Learn more.

Quote request

Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Concentration Price
EZ0018 1 mL 10 mg/mL 38 €
EZ0019 5 x 1 mL 10 mg/mL 154 €

Would you like to test a Free Sample? Please, inquiry at Free Sample Request.

We  also offer this Product with different specifications (GMP-grade is also available) or size (Custom Solutions), in higher amounts (Bulk Inquiries) or under OEM Basis (OEM Partnerships).

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Recombinant: bovine Pancreatic, purified from E. coli (29 kDa monomer).
  • High specific activity: more than 2,000 Kunitz units per mg protein.
  • High Quality: free of RNase and Proteinase contamination.
  • Complete solution: supplied with 10x Reaction Buffer.
  • Risk-free: product covered by our Quality 100% Guarantee.

Specifications

Unit definition:

One Kunitz unit is defined as the amount of enzyme required for the complete degradation of 1 µg of plasmid DNA in 10 minutes at 37 ˚C.

 

Note: One Kunitz unit equals to 50 Worthington units.

Includes

Includes :

– 1 mL DNase I (10 mg/mL)

– 1 mL Reaction Buffer (10x)

10x Reaction Buffer: 100 mM Tris-HCl (pH 7.5 at 25°C), 25 mM MgCl2, 1 mM CaCl2

Download documentation

Data Sheet
MSDS
Brochure

Applications

  • Removal of residual genomic DNA from RNA samples.
  • Degradation of DNA template in transcription reactions.
  • DNAse I footprinting.
  • Perform Nick Translation.

Quality Control

  • Functionally tested for digestion of template DNA after in vitro transcription.
  • Confirmed absence of RNAse activity.
  • Specific activity assayed by degradation of 1 µg of pUC18 in 40 mM Tris‐HCl (pH 8.0), 10 mM MgSO4, 1 mM CaCl2.

Storage, Shipping & Guarantee​

  • Shipped in: Gel pack.
  • Storage: -20 °C.
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Citations

Beatriz, M., Vilaça, R., Anjo, S. I., Manadas, B., Januário, C., Rego, A. C., & Lopes, C. (2022). Defective mitochondrial-lysosomal axis promotes extracellular vesicles release of mitochondrial components in Huntington’s Disease. bioRxiv.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Frequently asked questions

Do you have any question? Please visit our FAQs section GO

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