FastCONTROL™ p2RVc-lacZ/ ΔNGFR – Retroviral Dual Reporter
For a Faster, Convenient & Flexible Target Cell Transfection Control
Product Overview:
FastCONTROL™ Dual Reporters are fast, convenient and flexible target cell transfection control ideal for high co-expression of two reporter genes drive for ubiquitous, strong and constitutive promoters. FastCONTROL™ p2RVc-lacZ/ ΔNGFR Dual Reporter is a Retroviral vector that contains cytomegalovirus promoter (Pcmv) to drive the expression of both beta galactosidase (lacZ gene) and truncated nerve growth factor receptor (ΔNGFR). This is a self-inactivating (SIN) retroviral vector that lack viral promoter and enhancer activity in their 3′ long terminal repeat (LTR).
Availability: In stock! Ready-to-ship immediately
Catalog Numbers, Sizes & Prices
| Cat. No. | Size | Price |
| PC0128 | 15 µg | 188 € |
| PC0128-Plus | 15 µg + 0.2 mL | 214 € |
Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.
Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.
Advantages & Features
- Fast: available combination of 2 cell location markers in one vector.
- Convenient: available in Lentiviral, Retroviral or non-viral vectors, for immediate in vivo and in vitro expression.
- Compatible with transient or stable transfections.
- Flexible: available with different mammalian resistance markers and Dual Reporter genes combination for different cell location.
- Risk-free: product covered by our Quality 100% Guarantee.
Includes
Includes:
– 15 µL FastCONTROL™ Dual Reporter (1 µg/µL)
Plus Version – Includes:
– 15 µL FastCONTROL™ Dual Reporter (1 µg/µL)
– 0.2 mL CANFAST™ Transfection Reagent (1 mg/mL)
– 15 μL pASSEMBLE™ Viral Packaging Vector (1 μg/ μL)æ
æ ecotropic (tropism for rat or mouse cells) or amphotropic (tropism for most mammalian cells; hamster are not included).
Download documentation
Data SheetBrochureApplications
- Control for assessing the efficiency of transfection in mammalian cells.
- Targering of different cell locations.
- Obtention of cell lines with reporter proteins.
Tables, Figures & Protocols
Quality Control
- The quantity and quality of purified DNA attend to:
– Ratio 260/ 280 (1.8 – 2.0).
– Agarose gel Electrophoresis.
– Digestion with Restriction Endonucleases.
- Transient Transfection CHO-K1 (625 cells / well with these vectors) provides a SEAP activity >1,000 fold higher than untransfected cells themselves.
- The surface expression of NGFR in transiently transfected CHO > 60%.
- Expression of eGFP in transient intracellular CHO > 60%.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: FastCONTROL™ Dual Reporter Plasmid (-20 ºC) and CANFAST™ (4 ºC).
- Shelf Life: 24 months from the date of purchase, if it is properly stored.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Biosafety Features:
- Biosafety Level 2 (BL-2).
- One packaging vector.
- Disposable Packaging vectors are 3´LTS SIN and packaging signal deleted (ΔΨ).
- Viruses from SIN vectors results in the transcriptional inactivation of the provirus in the infected cell.
