Leucine-rich Repeat Containing G protein-coupled Receptor 5
ExpressMAX™ Expression-ready Human GPCR ORF Clones
Product Overview:
It is a Leucine-Rich Repeat-Containing Receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. It is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. It plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for the gene.
Quote requestAvailability: In stock! Ready-to-ship immediately
Catalog Numbers, Sizes & Prices
| Cat. No. | Size | Price |
| G0638-B | Bacterial Stock | 289 € |
| G0638-Plus | 15 µL (12-well plate) + 0.2 mL | 667 € |
Press here to learn the difference between this three products.
Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.
Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.
Advantages & Features
- Native structure.
- Wide range: A large collection of ExpressMAX™ GPCR ORF Clones availables.
- Highest levels of GPCR expression: on surface cell lines, > 50% of TAG detection by cytometry.
- Wide spectrum: of strong constitutive promoters.
- Complete solution: it contains all necessary elements for maximum receptor expression.
- Ready-to-use solution: avoids cloning steps, DNA ready to transfect.
- Easy protocol and detection: the whole procedure is simple, with minimal handling.
- Neomycin resistance.
- Ampicillin bacterial selection.
Specifications
- Plasmid size: 8637 pb
- Promoter: PEF1α
- ORF Sequence (GenBank based): BC096324
- Protein Sequence (SwissProt): O75473
- Bacterial Selection Antibiotics: Ampicillin
- Mammalian Selection Antibiotic: Neomycin
- Alternative names: LGR5, FEX or HG38
Includes
Includes:
ExpressMAX™ GPCR ORF Clones (Cat. No. – B)
– Vector as transformed Bacteria in stab culture or a Glycerol Stock format.
Mammalian Transfection Kits (Cat. No. – PLUS):
– 15 µg ExpressMAX™ Mammalian Expression Vector (1 mg/mL)
– 0.2 mL CANFAST™ Transfection Reagent
Download documentation
BrochureApplications
- Functional assays, as protein immobilization, cellular localization and other functional assays.
- High-throughput and large scale protein production and purification.
- Reverse transfection arrays and nucleic acid programmable protein arrays (NAPPA).
Quality Control
The quantity and quality of purified DNA is checked by:
- Ratio 260/ 280.
- Agarose gel electrophoresis.
- Digestion with restriction endonucleases.
Storage, Shipping & Guarantee
- Shipped in: Dry Ice.
- Storage: ExpressMAX™ GPCR ORF Clones (-20 ºC) and CANFAST™ (4 ºC).
- Shelf Life: 24 months from the date of purchase, if it is properly stored.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
