150 rxn = 15 rxn if processing 2 mL Blood Samples.

We  also offer this Product with different specifications or size (Custom Solutions), in higher amounts (Bulk Inquiries) or under OEM Basis (OEM Partnerships).

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

• Really easy and fast protocol: it takes 86 minutes to results.
• Highly efficient: 3-6 µg of genomic DNA from a 200 µL blood sample (30-90 μg of genomic DNA from a 2 mL).
• Versatile: high quality DNA obtained from different sources.
• Safe and convenient: avoids phenol/chloroform extraction.
• Pure genomic DNA: ready-to-use in all Molecular Biology applications.
• Reproducible extraction of high molecular weight genomic DNA purified.
• Risk-free: product covered by our Quality 100% Guarantee.

Includes for 150 rxn:

– 2 x 60 mL S1 Buffer

– 36 mL S2 Buffer

– 12 mL S3 Buffer

– 30 mg Proteinase K

– 10 mL EB Buffer

Purified DNA suitable for all common Molecular Biology applications, such as:

• PCR.
• Cloning.
• DNA sequencing.
• Southern blot analysis.

        Protocol:

– Tested on a lot-to-lot basis by isolating total DNA from 200 µL of whole human blood.

– DNA purified is analysed by:

• Spectrophotometer: Ratio 260/280 (1.6-1.8).
• Agarose gel Electrophoresis.

• Recommendations: If any kit reagent forms a precipitate, warm at 55–65 °C until the precipitate dissolves, and allow to cool to Room Temperature before use.

• Shipped in: Ambient Temperature.
• Storage: All components can be stored at Room Temperature, except Proteinase (4 ºC). Proteinase K should be stored at -20 ºC for longer-term storage. If any kit reagent forms a precipitate, warm at 55–65 °C until the precipitate dissolves, and allow to cool to room temperature before use.
• Shelf Life: All components are stable for 2 years,  if stored properly.

• González-Cano, R., Gonzalez-Martinez, A., Muñoz-Mejias, M. E., Valera, P., & Rodero, E. (2022). Removal to undesirable MC1R gene alleles from ‘Berrenda en Negro’, an endangered Spanish cattle breed, to enhance breed conservation programs. Livestock Science, 104844.
• Anaya, G., Molina, A., Valera, M., Moreno‐Millán, M., Azor, P., Peral‐García, P., & Demyda‐Peyrás, S. (2017). Sex chromosomal abnormalities associated with equine infertility: validation of a simple molecular screening tool in the Purebred Spanish Horse. Animal genetics, 48(4), 412-419.
• Dorado, J., Anaya, G., Bugno‐Poniewierska, M., Molina, A., Mendez‐Sanchez, A., Ortiz, I., … & Demyda‐Peyrás, S. (2017). First case of sterility associated with sex chromosomal abnormalities in a jenny. Reproduction in Domestic Animals, 52(2), 227-234.
• Fernández‐Bedmar, Z., Anter, J., Alonso‐Moraga, A., Martín de las Mulas, J., Millán‐Ruiz, Y., & Guil‐Luna, S. (2017). Demethylating and anti‐hepatocarcinogenic potential of hesperidin, a natural polyphenol of Citrus juices. Molecular carcinogenesis, 56(6), 1653-1662.

• Anaya Calvo-Rubio, G. (2017). Desarrollo y validación de un sistema diagnóstico rápido para la detección de alteraciones cromosómicas que provocan infertilidad en caballos basado en herramientas moleculares.
• Fernández-Bedmar, Z., Anter, J., de La Cruz-Ares, S., Muñoz-Serrano, A., Alonso-Moraga, Á., & Pérez-Guisado, J. (2011). Role of citrus juices and distinctive components in the modulation of degenerative processes: genotoxicity, antigenotoxicity, cytotoxicity, and longevity in Drosophila. Journal of Toxicology and Environmental Health, Part A, 74(15-16), 1052-1066.
• Juárez, M., Clemente, I., Polvillo, O., & Molina, A. (2009). Meat quality of tenderloin from Iberian pigs as affected by breed strain and crossbreeding. Meat science, 81(4), 573-579.
• Fernández‐Bedmar, Z., Anter, J., Alonso‐Moraga, A., Martín de las Mulas, J., Millán‐Ruiz, Y., & Guil‐Luna, S. (2017). Demethylating and anti‐hepatocarcinogenic potential of hesperidin, a natural polyphenol of Citrus juices. Molecular Carcinogenesis.
• Avilés, C., Horcada, A., Polvillo, O., Membrillo, A., Anaya, G., Molina, A., … & Panea, B. (2016). Association study between variability in the SCD gene and the fatty acid profile in perirenal and intramuscular fat deposits from Spanish goat populations. Small Ruminant Research, 136, 127-131.
• Mateo-Fernández, M., Merinas-Amo, T., Moreno-Millán, M., Alonso-Moraga, Á., & Demyda-Peyrás, S. (2016). In Vivo and In Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach. BioMed Research International, 2016.
• Anter, J., Romero-Jiménez, M., Fernández-Bedmar, Z., Villatoro-Pulido, M., Analla, M., Alonso-Moraga, A., & Muñoz-Serrano, A. (2011). Antigenotoxicity, cytotoxicity, and apoptosis induction by apigenin, bisabolol, and protocatechuic acid. Journal of medicinal food, 14(3), 276-283.
• Anter, J., Tasset, I., Demyda-Peyrás, S., Ranchal, I., Moreno-Millán, M., Romero-Jimenez, M., … & Alonso-Moraga, Á. (2014). Evaluation of potential antigenotoxic, cytotoxic and proapoptotic effects of the olive oil by-product “alperujo”, hydroxytyrosol, tyrosol and verbascoside. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 772, 25-33.
• Avilés, C., Polvillo, O., Peña, F., Juárez, M., Martínez, A. L., & Molina, A. (2013). Associations between gene polymorphisms and fat deposition in Spanish commercial beef. Journal of animal science, 91(10), 4571-4577.
• Polvillo, O., Juárez, M., Molina, A., Membrillo, A., & Clemente, I. Comparison of genetic (neutral DNA markers) and phenotypic (fresh meat quality traits) distances between Iberian pig strains.
• Pelayo, R., Valera, M., Molina, A., & Avilés, C. B. (2016). Short communication: Analysis of polymorphisms in candidate’s genes for meat quality in Lidia cattle. Spanish Journal of Agricultural Research, 14(4), 04-02.
• Rodero, E., González, A., Avilés, C., & Luque, M. (2013). Conservation of endangered Spanish cattle breeds using markers of candidate genes for meat quality. Animal biotechnology, 24(1), 15-24.
• Avilés, C., Peña, F., Polvillo, O., Barahona, M., Campo, M. M., Sañudo, C., … & Molina, A. (2015). Association between functional candidate genes and organoleptic meat traits in intensively-fed beef. Meat science, 107, 33-38.
• Clemente, I., Membrillo, A., Azor, P. J., Polvillo, O., Juárez, M., Santos, E., & Molina, A. (2008). Caracterización de la diversidad genética intrarracial del cerdo Ibérico. XIV Reunión Nacional de Mejora Genética Animal (2008),.
• Dorado, J., Anaya, G., Bugno‐Poniewierska, M., Molina, A., Mendez‐Sanchez, A., Ortiz, I., … & Demyda‐Peyrás, S. (2016). First case of sterility associated with sex chromosomal abnormalities in a jenny. Reproduction in Domestic Animals.
• Anaya, G., Molina, A., Valera, M., Moreno-Millán, M., Azor, P., Peral-García, P. and Demyda-Peyrás, S. (2017), Sex chromosomal abnormalities associated with equine infertility: validation of a simple molecular screening tool in the Purebred Spanish Horse. Anim Genet. doi:10.1111/age.12543
• Anter, J., Fernández-Bedmar, Z., Villatoro-Pulido, M., Demyda-Peyras, S., Moreno-Millán, M., Alonso-Moraga, Á., … & de Castro, M. D. L. (2011). A pilot study on the DNA-protective, cytotoxic, and apoptosis-inducing properties of olive-leaf extracts. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 723(2), 165-170.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.