HigherPurity™ Blood DNA Extraction Kit

For a Reliable, Easy & High-Quality isolation of DNA from 200 μl – 2mL Whole Blood, Buffy Coat or Cultured Cells

HigherPurity™ Blood DNA Extraction Kit  is a reliable, easy-to-use and rapid method for high-quality genomic DNA Purification from various sources, including: whole blood, buffy coat or cultured cells. The procedure includes: lysis, protein removal, DNA precipitation, washing and hydration.

Price: 111.82 229.11 

Detailed information:

Advantages & Features
  • Highly efficient: 100-250 μg of Genomic DNA from a 5 mL Blood sample (150-500 μg from a 10 mL).
  • Versatile: high quality DNA obtained from different sources.
  • Safe and convenient: avoids phenol/chloroform extraction.
  • Pure genomic DNA: ready-to-use in all Molecular Biology applications.
Specifications
Includes

Includes for 50 rxn:
– 40 mL S1 Buffer
– 12 mL S2 Buffer
– 5 mL S3 Buffer
– 11 mg Proteinase K
– 5 mL EB Buffer

 

Download documentation
Datasheet
MSDS
Applications

Purified DNA suitable for all common Molecular Biology applications, such as:

  • PCR.
  • Cloning.
  • DNA sequencing.
  • Southern blot analysis.
Tables & Figures

Quality Control

– Tested on a lot-to-lot basis by isolating total DNA from 5 mL of whole Human Blood.
– DNA purified is analysed by:

• Spectrophotometer: Ratio 260/ 280 (1.6-1.8).
• Agarose gel Electrophoresis.

Advice
Storage, Shipping & Guarantee
  • Shipped in: Ambient Temperature.
  • Storage: All components can be stored at Room Temperature, except Proteinase (4 ºC). Proteinase K should be stored at -20 ºC for longer-term storage. If any kit reagent forms a precipitate, warm at 55–65 °C until the precipitate dissolves, and allow to cool to room temperature before use.
Citations
  • González-Cano, R., Gonzalez-Martinez, A., Muñoz-Mejias, M. E., Valera, P., & Rodero, E. (2022). Removal to undesirable MC1R gene alleles from ‘Berrenda en Negro’, an endangered Spanish cattle breed, to enhance breed conservation programs. Livestock Science, 104844.
  • Pirosanto, Y., et al. “Screening and detection of chromosomal copy number alterations in the domestic horse using SNP‐array genotyping data.” Animal Genetics (2021).
  • Anaya, G., Fernández, M. E., Valera, M., Molina, A., Azcona, F., Azor, P., … & Demyda-Peyrás, S. (2018). Prevalence of twin foaling and blood chimaerism in purebred Spanish horses. The Veterinary Journal234, 142-144
  • Anaya, G., Molina, A., Valera, M., Moreno‐Millán, M., Azor, P., Peral‐García, P., & Demyda‐Peyrás, S. (2017). Sex chromosomal abnormalities associated with equine infertility: validation of a simple molecular screening tool in the Purebred Spanish Horse. Animal genetics48(4), 412-419.
  • Dorado, J., Anaya, G., Bugno‐Poniewierska, M., Molina, A., Mendez‐Sanchez, A., Ortiz, I., … & Demyda‐Peyrás, S. (2017). First case of sterility associated with sex chromosomal abnormalities in a jenny. Reproduction in Domestic Animals52(2), 227-234.
  • Fernández‐Bedmar, Z., Anter, J., Alonso‐Moraga, A., Martín de las Mulas, J., Millán‐Ruiz, Y., & Guil‐Luna, S. (2017). Demethylating and anti‐hepatocarcinogenic potential of hesperidin, a natural polyphenol of Citrus juices. Molecular carcinogenesis56(6), 1653-1662.
Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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