Horse-Power™ Green-Taq DNA Polymerase
Highly Purified (>98%) with Green Buffer (10x) for Save your time: direct from PCR to Gel Electrophoresis
Product Overview:
Horse-Power™ Green-Taq DNA Polymerase is a pure, versatile and thermostable recombinant enzyme produced in an E. coli strain, which carries the cloned pol gene from Thermus aquaticus. The enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading).
10x Green Buffer contains an Agarose Loading Buffer including two tracking dyes (blue and yellow dye) for visual tracking of DNA migration and a dense compound to facilitate the drop-down of the samples into the well Agarose gels. Blue dye (migrates with 3 to 5 kb DNA fragments in 1% agarose gel) and Yellow dye (migrates faster than 10 bp DNA fragments in 1% Agarose gel).
Quote requestAvailability: In stock! Ready-to-ship immediately
Catalog Numbers, Sizes & Prices
| Cat. No. | Size | Price |
| P0023-GT | 500 U | 51 € |
We also offer this Product with different specifications or size (Custom Solutions), in higher amounts (Bulk Inquiries) or under OEM Basis (OEM Partnerships).
Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.
Advantages & Features
- Highest purity: greater than 98% confirmed by SDS-PAGE.
- Highest quality: high activity, specificity, thermostability and performance in PCR.
- Highly efficient: reactivation buffer improved.
- Save Time: go directly from PCR to Gel Electrophoresis.
- Thermostable: half-life at 94 ºC is 40 minutes.
- Adds extra nucleotides: preferentially adenine, without template at 3´ends leaving 3´overhangs PCR fragments.
- Incorporates modified nucleotides: biotinylated, fluorescently labelled, etc.
- Molecular Weight: 94 kDa.
- Risk-free: product covered by our Quality 100% Guarantee.
Specifications
Assay conditions:
25 mM Tris-HCl pH 9.0 at 25 °C, 50 mM KCl, 2 mM MgCl2, 0.1 mg/mL gelatine, 200 µM dATP, dGTP, dTTP, 100 µM [α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA.
Unit definition:
One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.
Includes
Includes:
– 100 μL Horse-Power™ Taq DNA Polymerase (5 U/μL)
– 2 x 1.25 mL Green Buffer (10x) with 20 mM MgCl2
Download documentation
Data SheetApplications
- Routine amplifications.
- Colony screening.
- Amplifications up to 6 kb using Plasmid, Viral or Genomic DNA as template.
- PCR fragments amplification for TA or GC Cloning.
Quality Control
- Functionally tested in PCR.
- Undetected bacterial DNA (by PCR).
- Undetectable nucleases (endo-, exo, and ribonucleases) activities guaranteed by appropriate quality tests.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
- Shelf Life: 24 months from the date of purchase, if it is properly stored.
Citations
- Peykov, S., Strateva, T., & Dimov, S. (2022). Design of Species-Specific PCR Primers That Target the aac (6′)-Ii Gene for the Rapid Detection of Enterococcus faecium. Bacteria, 1(3), 183-190.
- Bañón, Rafael, et al. «Exploring deep-sea biodiversity in the porcupine bank (NE Atlantic) through fish integrative taxonomy.» Journal of Marine Science and Engineering 9.10 (2021): 1075.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
