We also offer this Product with different specifications or size (Custom Solutions), in higher amounts (Bulk Inquiries) or under OEM Basis (OEM Partnerships).

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

• Highest purity: greater than 98% confirmed by SDS-PAGE.
• Highest quality: high activity, specificity, thermostability and performance in PCR.
• Highly efficient: reactivation buffer improved.
• Thermostable: half-life at 94 ºC is 40 minutes.
• Adds extra nucleotides: preferentially adenine, without template at 3´ends leaving 3´overhangs PCR fragments.
• Incorporates modified nucleotides: biotinylated, fluorescently labelled, etc.
• Molecular Weight: 94 kDa.
• Convenient: available in different concentrations, sizes and solutions.
• Risk-free: product covered by our Quality 100% Guarantee.

Assay conditions:

25 mM Tris-HCl pH 9.0 at 25 °C, 50 mM KCl, 2 mM MgCl₂, 0.1 mg/mL gelatine, 200 µM dATP, dGTP, dTTP, 100 µM [α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA.

Unit definition:

One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.

Includes for 1.25 mL:

– 2 x 1.25 mL Horse-Power™ Taq DNA polymerase MasterMix (2x) (2 U/μL)

• Routine amplifications.
• Colony screening (see Horse-Power™ Red-Taq DNA Polymerase).
• Amplifications up to 5 kb using plasmid, viral or genomic DNA as template.
• PCR fragments amplification for TA or GC cloning.

• Functionally tested in PCR.
• Free of bacterial DNA (by qPCR).
• Exempt of nucleases (endo-, exo and ribonucleases) activities guaranteed by appropriate quality tests.

• Shipped in: Gel pack.
• Storage: -20 °C (NON Frost-Free Freezer).
• Shelf Life: 24 months from the date of purchase, if it is properly stored.

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• Arjona-López, J. M., & López-Herrera, C. J. (2020). Entoleuca sp. infected by mycoviruses as potential biocontrol agents of avocado white root rot. European Journal of Plant Pathology, 1-12.
• Porcellato, I., Mecocci, S., Mechelli, L., Cappelli, K., Brachelente, C., Pepe, M., … & Modesto, P. (2020). Equine Penile Squamous Cell Carcinomas as a Model for Human Disease: A Preliminary Investigation on Tumor Immune Microenvironment. Cells, 9(11), 2364.
• Hatzilazarou, S., Kostas, S., Joachim, M., & Economou, A. (2020). Regeneration of Viburnum dentatum L. from Alginate-Encapsulated Shoot Explants after Short-Term Cold Storage and Assessment of Genetic Stability Using ISSR Analysis. Agronomy, 10(11), 1660.
• RUSENOVA, N., KRUSTEV, S., ATANASOV, A., RUSENOV, A., & STANILOVA, S. Differentiation of Staphylococcus pseudintermedius in the Staphylococcus intermedius Group (SIG) by Conventional and Molecular Methods. thought, 10, 11.
• Arjona-López, J. M., Capote, N., & López-Herrera, C. J. (2019). Improved real-time PCR protocol for the accurate detection and quantification of Rosellinia necatrix in avocado orchards. Plant and Soil, 1-8.
• Fagín, E., Bravo, I., Garrido, J. L., Rodríguez, F., & Figueroa, R. I. Scrippsiella acuminata versus Scrippsiella ramonii: A Physiological Comparison. Cytometry Part A.
• Ortiz‐Bustos, C. M., López‐Bernal, A., Testi, L., & Molinero‐Ruiz, L. Environmental and irrigation conditions can mask the effect of Magnaporthiopsis maydis on growth and productivity of maize. Plant Pathology.
• SERRA MARTÍNEZ,. (2017). Detección de posibles fuentes de inoculo de Fusicladium eriobotryae en níspero.
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• Ruiz-Ruano, F. J., Castillo-Martínez, J., Cabrero, J., Gómez, R., Camacho, J. P. M., & López-León, M. D. (2018). High-throughput analysis of satellite DNA in the grasshopper Pyrgomorpha conica reveals abundance of homologous and heterologous higher-order repeats. Chromosoma, 127(3), 323-340.
• Aguilar, L., Gallegos, Á., Arias, C. A., Ferrera, I., Sánchez, O., Rubio, R., … & Pérez, C. (2019). Microbial nitrate removal efficiency in groundwater polluted from agricultural activities with hybrid cork treatment wetlands. Science of the total environment, 653, 723-734.

• Robledo-Mahón, T., Silva-Castro, G. A., Kuhar, U., Jamnikar-Ciglenečki, U., Barlič-Maganja, D., Aranda, E., & Calvo, C. (2019). Effect of Composting Under Semipermeable Film on the Sewage Sludge Virome. Microbial ecology, 1-9.
• Negreiros, A. M. P., Sales Júnior, R., León, M., Melo, N. J. D. A., Michereff, S. J., Ambrósio, M. M. D. Q., … & Armengol, J. Identification and pathogenicity of Macrophomina species collected from weeds in melon fields in Northeastern Brazil. Journal of Phytopathology.
• Rêgo, T. J. S., Elena, G., Correia, K. C., Tovar‐Pedraza, J. M., Câmara, M. P. S., Armengol, J., … & Berbegal, M. Genetic diversity and population structure of Lasiodiplodia theobromae from different hosts in northeastern Brazil and Mexico. Plant Pathology.
• Robledo Mahón, T. (2018). Estudio de los procesos biológicos y de la estructura de las comunidades microbianas en el proceso de compostaje de lodos de depuradora de aguas residuales urbanas, en sistemas de membranas semipermea.
• Mora-Sala, B., Cabral, A., León, M., Agustí-Brisach, C., Armengol, J., & Abad-Campos, P. Survey, Identification and Characterization of cylindrocarpon-like asexual morphs in Spanish forest nurseries. Plant Disease, (ja).
• Robledo-Mahón, T., Aranda, E., Pesciaroli, C., Rodríguez-Calvo, A., Silva-Castro, G. A., González-López, J., & Calvo, C. (2018). Effect of semi-permeable cover system on the bacterial diversity during sewage sludge composting. Journal of Environmental Management, 215, 57-67.
• Rodriguez-Sanchez, A., Margareto, A., Robledo-Mahon, T., Aranda, E., Diaz-Cruz, S., Gonzalez-Lopez, J., … & Gonzalez-Martinez, A. (2017). Performance and bacterial community structure of a granular autotrophic nitrogen removal bioreactor amended with high antibiotic concentrations. Chemical Engineering Journal, 325, 257-269.
• Ruiz-Estévez, M., Bakkali, M., Martín-Blázquez, R., & Garrido-Ramos, M. A. (2017). Identification and Characterization of TALE Homeobox Genes in the Endangered Fern Vandenboschia speciosa. Genes, 8(10), 275.
• Ruiz-Estévez, M., Bakkali, M., Martín-Blázquez, R., & Garrido-Ramos, M. A. (2017). Differential expression patterns of MIKCC-type MADS-box genes in the endangered fern Vandenboschia speciosa. Plant Gene, 12, 50-56.
• Navarro-Domínguez, B., Ruiz-Ruano, F. J., Camacho, J. P. M., Cabrero, J., & López-León, M. D. (2017). Transcription of a B chromosome CAP-G pseudogene does not influence normal Condensin Complex genes in a grasshopper. Scientific reports, 7(1), 17650.
• Núñez Delegido, E. (2017). Caracterización funcional de los genes CRD de Arabidopsis thaliana.
• Rodriguez-Sanchez, A., Margareto, A., Robledo-Mahon, T., Aranda, E., Diaz-Cruz, S., Gonzalez-Lopez, J., … & Gonzalez-Martinez, A. (2017). Performance and bacterial community structure of a granular autotrophic nitrogen removal bioreactor amended with high antibiotic concentrations. Chemical Engineering Journal.
• Anaya Calvo-Rubio, G. (2017). Desarrollo y validación de un sistema diagnóstico rápido para la detección de alteraciones cromosómicas que provocan infertilidad en caballos basado en herramientas moleculares.
• Calvo‐Polanco, M., Sánchez‐Castro, I., Cantos, M., García, J. L., Azcón, R., Ruiz‐Lozano, J. M., … & Aroca, R. (2016). Effects of different arbuscular mycorrhizal fungal backgrounds and soils on olive plants growth and water relation properties under well‐watered and drought conditions. Plant, Cell & Environment, 39(11), 2498-2514.
• Núñez Delegido, E. (2017). Caracterización funcional de los genes CRD de Arabidopsis thaliana.
• León-Ortega, M., & González-Wangüemert, M. (2015). Characterization of 10 new tetranucleotide microsatellite markers for the European eagle owl, Bubo bubo: Useful tools for conservation strategies. Biochemical Systematics and Ecology, 63, 109-112.
• Demyda-Peyrás, S., Anaya, G., Bugno-Poniewierska, M., Pawlina, K., Membrillo, A., Valera, M., & Moreno-Millán, M. (2014). The use of a novel combination of diagnostic molecular and cytogenetic approaches in horses with sexual karyotype abnormalities: a rare case with an abnormal cellular chimerism. Theriogenology, 81(8), 1116-1122.
• Navarro-Domínguez, B., Cabrero, J., Camacho, J. P. M., & López-León, M. D. (2016). B-chromosome effects on Hsp70 gene expression does not occur at transcriptional level in the grasshopper Eyprepocnemis plorans. Molecular Genetics and Genomics, 291(5), 1909-1917.
• ALMENDROS, V. M. T. (2016). Identificación de especies de Dactylonectria e Ilyonectria asociadas al pie negro de la vid en Castilla la Mancha.
• Martínez-Miguel, P., Valdivielso, J. M., Medrano-Andrés, D., Román-García, P., Cano-Peñalver, J. L., Rodríguez-Puyol, M., … & López-Ongil, S. (2014). The active form of vitamin D, calcitriol, induces a complex dual upregulation of endothelin and nitric oxide in cultured endothelial cells. American Journal of Physiology-Endocrinology and Metabolism, 307(12), E1085-E1096.
• Daddaoua, A., Molina-Santiago, C., de la Torre, J., Krell, T., & Ramos, J. L. (2014). GtrS and GltR form a two-component system: the central role of 2-ketogluconate in the expression of exotoxin A and glucose catabolic enzymes in Pseudomonas aeruginosa. Nucleic acids research, 42(12), 7654-7665.
• Nieves-Morión, M., Lechno-Yossef, S., López-Igual, R., Frías, J. E., Mariscal, V., Nürnberg, D. J., … & Flores, E. (2017). Specific glucoside transporters influence septal structure and function in the filamentous, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. Journal of Bacteriology, JB-00876.
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• Mohammadi, H., Banihashemi, Z., Gramaje, D., & Armengol, J. (2012). Fungal pathogens associated with grapevine trunk diseases in Iran. Journal of Agricultural Science and Technology, 15(1), 137-150.
• Hernández-Hernández, J., Espino, A., Rodríguez-Rodríguez, J. M., Pérez-Sierra, A., León, M., Abad-Campos, P., & Armengol, J. (2010). Survey of diseases caused by Fusarium spp. on palm trees in the Canary Islands. Phytopathologia Mediterranea, 49(1), 84-88.
• Boughalleb, N., Salem, I. B., Beltrán, R., Vicent, A., Sierra, A. P., Abad‐Campos, P., … & Armengol, J. (2010). Occurrence of Monosporascus cannonballus in watermelon fields in Tunisia and factors associated with ascospore density in soil. Journal of phytopathology, 158(3), 137-142.
• Soto, E. C., Yáñez-Ruiz, D. R., Cantalapiedra-Hijar, G., Vivas, A., & Molina-Alcaide, E. (2012). Changes in ruminal microbiota due to rumen content processing and incubation in single-flow continuous-culture fermenters. Animal production science, 52(9), 813-822.
• Jroundi, F., Fernández-Vivas, A., Rodriguez-Navarro, C., Bedmar, E. J., & González-Muñoz, M. T. (2010). Bioconservation of deteriorated monumental calcarenite stone and identification of bacteria with carbonatogenic activity. Microbial ecology, 60(1), 39-54.
• Macias, M. I., Grande, J., Moreno, A., Domínguez, I., Bornstein, R., & Flores, A. I. (2010). Isolation and characterization of true mesenchymal stem cells derived from human term decidua capable of multilineage differentiation into all 3 embryonic layers. American journal of obstetrics and gynecology, 203(5), 495-e9.
• Clemente, A., Marín-Manzano, M. C., Jiménez, E., Arques, M. C., & Domoney, C. (2012). The anti-proliferative effect of TI1B, a major Bowman–Birk isoinhibitor from pea (Pisum sativum L.), on HT29 colon cancer cells is mediated through protease inhibition. British Journal of Nutrition, 108(S1), S135-S144.
• Alvarez, L. A., León, M., Abad-Campos, P., García-Jiménez, J., & Vicent, A. (2011). Genetic variation and host specificity of Phytophthora citrophthora isolates causing branch cankers in Clementine trees in Spain. European journal of plant pathology, 129(1), 103-117.
• Pérez-Sierra, A., León, M., Álvarez, L. A., Alaniz, S., Berbegal, M., García-Jiménez, J., & Abad-Campos, P. (2010). Outbreak of a new Phytophthora sp. associated with severe decline of almond trees in eastern Spain. Plant Disease, 94(5), 534-541.
• Jiménez-Hidalgo, M., Kurz, C. L., Pedrajas, J. R., Naranjo-Galindo, F. J., González-Barrios, M., Cabello, J., … & Fierro-González, J. C. (2014). Functional characterization of thioredoxin 3 (TRX-3), a Caenorhabditis elegans intestine-specific thioredoxin. Free Radical Biology and Medicine, 68, 205-219.
• Berbegal, M., Pérez-Sierra, A., Armengol, J., & Grünwald, N. J. (2013). Evidence for multiple introductions and clonality in Spanish populations of Fusarium circinatum. Phytopathology, 103(8), 851-861.
• Alaniz, S., Armengol, J., León, M., García-Jiménez, J., & Abad-Campos, P. (2009). Analysis of genetic and virulence diversity of Cylindrocarpon liriodendri and C. macrodidymum associated with black foot disease of grapevine. Mycological research, 113(1), 16-23.
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• Ortiz-Bustos, C. M., Testi, L., García-Carneros, A. B., & Molinero-Ruiz, L. (2016). Geographic distribution and aggressiveness of Harpophora maydis in the Iberian peninsula, and thermal detection of maize late wilt. European Journal of Plant Pathology, 144(2), 383-397.
• Soto, E. C., Molina-Alcaide, E., Khelil, H., & Yáñez-Ruiz, D. R. (2013). Ruminal microbiota developing in different in vitro simulation systems inoculated with goats’ rumen liquor. Animal Feed Science and Technology, 185(1), 9-18.
• Rodríguez-Ruano, S. M., Martín-Vivaldi, M., Martín-Platero, A. M., López-López, J. P., Peralta-Sánchez, J. M., Ruiz-Rodríguez, M., … & Martínez-Bueno, M. (2015). The Hoopoe’s uropygial gland hosts a bacterial community influenced by the living conditions of the bird. PloS one, 10(10), e0139734.
• Armengol, J., Vicent, A., León, M., Berbegal, M., Abad‐Campos, P., & García‐Jiménez, J. (2010). Analysis of population structure of Rosellinia necatrix on Cyperus esculentus by mycelial compatibility and inter‐simple sequence repeats (ISSR). Plant pathology, 59(1), 179-185.
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• González‐Domínguez, E., León, M., Armengol, J., & Berbegal, M. (2015). A Nested Polymerase Chain Reaction Protocol for in planta Detection of Fusicladium eriobotryae, Causal Agent of Loquat Scab. Journal of Phytopathology, 163(5), 415-418.
• Tello, M. L., Gramaje, D., Gómez, A., Abad-Campos, P., & Armengol, J. (2010). Analysis of phenotypic and molecular diversity of Phaeomoniella chlamydospora isolates in Spain. Journal of Plant Pathology, 195-203.
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• González, M., Pérez‐Sierra, A., Serrano, M. S., & Sánchez, M. E. (2016). Two Phytophthora species causing decline of wild olive (Olea europaea subsp. europaea var. sylvestris). Plant Pathology.
• Fernández-Bedmar, Z., & Alonso-Moraga, A. (2016). In vivo and in vitro evaluation for nutraceutical purposes of capsaicin, capsanthin, lutein and four pepper varieties. Food and Chemical Toxicology, 98, 89-99.
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• Agustí-Brisach, C., Cabral, A., González-Domínguez, E., Pérez-Sierra, A., León, M., Abad-Campos, P., … & Armengol, J. (2016). Characterization of Cylindrodendrum, Dactylonectria and Ilyonectria isolates associated with loquat decline in Spain, with description of Cylindrodendrum alicantinum sp. nov. European Journal of Plant Pathology, 145(1), 103-118.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.