HotBegan™ Hot Start Taq DNA Polymerase MasterMix

HotBegan™ Hot Start Taq DNA Polymerase MasterMix

For a Specific, Highly Efficient & Reliable Amplifications of DNA up 160 fg in Ready-to-use Format

Product Overview:

HotBegan™ Hot Start Taq DNA Polymerase MasterMix (2x) an optimized ready-to-use solution containing HotBegan Taq DNAPolymerase (hot start performance), dNTPs, MgCl2 and Stabilizers. It is inactive at Room Temperature and includes all material needed except Template and Primers.

It is a Horse-Power™ Taq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until a denaturation step occurs. The heat-labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and non-specific products.

Like Horse-Power™ Taq DNA Polymerase, the enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Before enzyme activation none of the enzyme activities are detectable.

Quote request

Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Concentration Price
P0030 2 x 1.25 mL (250 rxn) MasterMix (2x) 61 €

 

We also offer this Product with different specifications or size (Custom Solutions), in higher amounts (Bulk Inquiries) or under OEM Basis (OEM Partnerships).

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Time-saving solution: ready-to-use MasterMix.
  • High specificity: minimizes unspecific amplification.
  • Efficient: prevents or minimizes primer-dimer and nonspecific products.
  • Great sensitivity: amplifies from femptograms of DNA targets.
  • Inactive: at Room Temperature.
  • Optimized: adds extra nucleotides (preferentially adenine) without template at 3´ends leaving 3´overhangs PCR fragments.
  • Powerful: amplification of targets up to 5 kb.
  • Convenient: available in kits and MasterMix solution.
  • Risk-free: product covered by our Quality 100% Guarantee.

Specifications

Unit definition:

One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74°C.

Includes

Includes:

– 2 x 1.25 mL HotBegan™ Hot Start Taq DNA Polymerase MasterMix, which includes HotBegan™ Hot Start Taq DNA Polymerase (0.1 U/μL), 2x PCR Buffer, 0.4 mM of each dNTP, 4 mM Mg2+, 4% Glycerol.
– 2 x 1.25 mL Nuclease-free water.

Download documentation

Data Sheet

Applications

  • Real time PCR.
  • RT-PCR and quantitative RT-PCR.
  • Genotyping with Taqman Probes.
  • PCR fragments amplification for TA or GC Cloning.
  • Amplification from a limited DNA template or low copy number genes.

Quality Control

  • Functionally tested in qPCR.
  • None detected bacterial DNA (by qPCR).
  • Exempt of nucleases (endo-, exo and ribonucleases) activity guaranteed by appropriate quality tests.

Storage, Shipping & Guarantee​

  • Shipped in: Gel pack.
  • Storage: -20 °C.
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Citations

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Current Promotions


AgaPure™ Agarose Standard: 181€ (500g) Learn more Here


BrightMAX™ DNA Ladders from 39 € (50 µg) Learn more Here


TBARS Assay Kit from 229€ (96 assays) Learn more Here


UltraPure Water, DNASE/RNASE – Free from 34€ (1 L) Learn more Here


qMAXSen™ One-Step RT-qPCR Kits from 144 € (100 rxn) Learn more Here


XTT Cell Proliferation Assay Kit from 91 € (500 assays) Learn more Here


HigherPurity™ PCR Clean-Up Magnetic Beads: 261€ (25 mL) Learn more Here


Horse-Power™ Taq DNA Polymerase: 39 € (500 U) Learn more Here


GreenSafe DNA Gel Stain: 99 € (1 mL) Learn more Here


Horse-Power™ Taq DNA Polymerase (500 U) from 39€ Learn more Here


Stool Sample Collection and Stabilization Kit: 392 € (100 units) Learn more Here


IPTG: 34 € (5 gr) Learn more Here


PRImeZOL™ Reagent: 84 € (100 mL) Learn more Here


Proteinase K (Lyophilized): 79 € (100 mg) Learn more Here


Saliva Sample Collection Kit: 755 € (100 units) Learn more Here


qMAXSen™ qPCR MasterMixes from 117 € (250 rxn) Learn more Here


CVX-Mag™ Viral RNA Extraction Kit, CE-IVD: 336 € (100 rxn) Learn more Here


TruePure™ dNTP Mix, 1mL from 23€ Learn more Here


Resazurin Cell Viability Assay from 92 € (2,500 assays) Learn more Here


CVX™ Viral RNA Extraction Kit, CE-IVD: 336 € (100 rxn) Learn more Here

Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

Leave a Reply

You must be logged in to post a comment.

  • slidebg1

    Related products

    Horse-Power™ Taq DNA Polymerase

    Horse-Power™ Taq DNA Polymerase is a pure, versatile and thermostable recombinant enzyme produced in an E. coli strain, which carries the cloned pol gene from Thermus aquaticus. The enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but...


    Learn more
  • slidebg1

    Related products

    Horse-Power™ Taq DNA Polymerase MasterMix (2x)

    Horse-Power™ Taq DNA Polymerase MasterMix (2x) is an optimized ready-to-use MasterMix that contains all PCR reaction components: dNTPs, PCR buffer, Mg2+ and Taq DNA Polymerase. Only primers and template need to be added. The convenient MasterMix (2x) formulation saves time and eliminates the risk of contamination...


    Learn more
  • slidebg1

    Related products

    Horse-Power™ Taq DNA Polymerase with TruePure™ dNTPs

    Horse-Power™ Taq DNA Polymerase is a pure, versatile and thermostable recombinant enzyme produced in an E. coli strain, which carries the cloned pol gene from Thermus aquaticus. The enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but...


    Learn more
  • slidebg1

    Related products

    HotBegan™ Hot Start Taq DNA Polymerase

    HotBegan™ Hot Start Taq DNA Polymerase is a specific, highly efficient and sensitive HotStart DNA Polymerase designed to minimize unspecific amplification, improving PCR specificity. It is a Horse-Power™ Taq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until...


    Learn more