HotBegan™ Hot Start Taq DNA Polymerase MasterMix

HotBegan™ Hot Start Taq DNA Polymerase MasterMix

For a Specific, Highly Efficient & Reliable Amplifications of DNA up 160 fg in Ready-to-use Format

Product Overview:

HotBegan™ Hot Start Taq DNA Polymerase MasterMix (2x) an optimized ready-to-use solution containing HotBegan Taq DNAPolymerase (hot start performance), dNTPs, MgCl2 and Stabilizers. It is inactive at Room Temperature and includes all material needed except Template and Primers.

It is a Horse-Power™ Taq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until a denaturation step occurs. The heat-labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and non-specific products.

Like Horse-Power™ Taq DNA Polymerase, the enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Before enzyme activation none of the enzyme activities are detectable.

Quote request

Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No.SizeConcentrationPrice
P00302 x 1.25 mL (250 rxn)MasterMix (2x)59 €

Would you like to test a Free Sample? Please, inquiry at Free Sample Request.

We also offer this Product with different specifications or size (Custom Solutions), in higher amounts (Bulk Inquiries) or under OEM Basis (OEM Partnerships).

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Time-saving solution: ready-to-use MasterMix.
  • High specificity: minimizes unspecific amplification.
  • Efficient: prevents or minimizes primer-dimer and nonspecific products.
  • Great sensitivity: amplifies from femptograms of DNA targets.
  • Inactive: at Room Temperature.
  • Optimized: adds extra nucleotides (preferentially adenine) without template at 3´ends leaving 3´overhangs PCR fragments.
  • Powerful: amplification of targets up to 5 kb.
  • Convenient: available in kits and MasterMix solution.
  • Risk-free: product covered by our Quality 100% Guarantee.

Specifications

Unit definition:

One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74°C.

Includes

Includes:

– 2 x 1.25 mL HotBegan™ Hot Start Taq DNA Polymerase MasterMix, which includes HotBegan™ Hot Start Taq DNA Polymerase (0.1 U/μL), 2x PCR Buffer, 0.4 mM of each dNTP, 4 mM Mg2+, 4% Glycerol.
– 2 x 1.25 mL Nuclease-free water.

Download documentation

Data Sheet

Applications

  • Real time PCR.
  • RT-PCR and quantitative RT-PCR.
  • Genotyping with Taqman Probes.
  • PCR fragments amplification for TA or GC Cloning.
  • Amplification from a limited DNA template or low copy number genes.

Quality Control

  • Functionally tested in qPCR.
  • None detected bacterial DNA (by qPCR).
  • Exempt of nucleases (endo-, exo and ribonucleases) activity guaranteed by appropriate quality tests.

Storage, Shipping & Guarantee​

  • Shipped in: Gel pack.
  • Storage: -20 °C.
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Citations

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

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