mRNA Cap 2-O-Methyltransferase

mRNA Cap 2-O-Methyltransferase

High Quality & Purity Enzyme manufactured from Recombinant source

Product Overview:

Recombinant mRNA Cap 2′-O-Methyltransferase from Vaccinia virus is produced in Escherichia coli. It catalyzes the addition of a methyl group at the 2′-O position of the first nucleotide adjacent to the cap structure at the 5′ end of the RNA. The enzyme utilizes SAM as a methyl donor to methylate capped RNA (Cap 0) resulting in a cap-1 structure.

 

GMP-grade is also available. Learn more

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Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Concentration Price
EZ0047 2,000U 50 U/µL 52 €

 

We  also offer this Product with different specifications (GMP-grade is also available) or size (Custom Solutions), in higher amounts (Bulk Inquiries) or under OEM Basis (OEM Partnerships).

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Isolated form a recombinant source.
  • Extensivelly quality controlled: Endo/Exonucleases/RNAses free.
  • Flexible Quality Systems that covers from RUO to cGMP.

Specifications

Protein information
Predicted MW: 39 kDa
Purity: ≥ 95% as determined by SDS-polyacrylamide gels with Coomassie®blue staining.
Buffer Storage: 100 mM NaCl, 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1 % Triton®X-100.
Biological activity: One unit is defined as the amount of enzyme required to methylate 10 pmoles of 80 nt long capped RNA transcript in 1 hour at 37°C.

Gene Information
Synonyms: Poly(A) polymerase regulatory subunit, Poly(A) polymerase small subunit (PAP-S), VP39
Official Symbol: PAPS
Specie: Bovine Vaccinia virus
GenBank accession number: AY243312.1
Protein RefSeq: P07617
Protein Family: Methyl transferase

Includes

Includes:

– 2,000 U mRNA Cap 2′-O-Methyltransferase (50 U/µl)
– S-adenosylmethionine (SAM)
– Capping Buffer (10x)

Download documentation

Data Sheet
Brochure

Applications

  • 2´-O-methylation of capped in vitro transcripts helps the RNA evade innate immune response in vivo.

Quality Control

Quality Tests for Research Use Only version includes: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, etc.

Request your CoA >

Advice

Avoid exposure to constant temperature changes.

Storage, Shipping & Guarantee​

  • Shipped in: Gel Pack.
  • Storage: -20 ºC (NON Frost-Free Freezer).

Safety Statements

This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

To be informed about our Safety Staments for GMP Products, please contact us.

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Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

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