One-Step RT PCR Kit

One-Step RT PCR Kit

Highly Efficient cDNA Synthesis and PCR in a convenient Single Tube

Product Overview:

One-Step RT PCR Kit is designed for a Highly Efficient cDNA Synthesis and PCR in a single tube. The kit includes a PCR MasterMix supplied in a 2x concentration to perform standard PCR.

MasterMix contains all the reagents needed for running PCR reactions (except PCR primers and template). In addition, a separate RT Mix composed by a balanced mixture of both RTase and RNase Inhibitor is also provided.

RT-PCR is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the Reverse Transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR step synthesis, Taq DNA Polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. During subsequent rounds of cycling the DNA Polymerase exponentially amplifies the double-stranded DNA template.

 

Quote request

Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No.SizePrice
P0062100 rxn 254 €
P0063500 rxn799 €

Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Higher specificity, sensitivity, and yield.
  • Efficient: thermostable Reverse Transcriptase and RNase Inhibitor providing high cDNA yields.
  • Unique: Hot Start Taq DNA Polymerase in a mix with high-quality dNTPs.
  • Highly Sensitive: PCR enhancers allowing sensitive low background amplification.

Includes

Includes for 100 rxn:

– 2 x 1.25 mL One-Step PCR Mix (2x)
– 2 x 125 μL RT Mix
– 2 mL Water (RNase-free)

Download documentation

Data Sheet

Applications

  • One-step RT-PCR.
  • Amplification of GC-rich and complex templates.

Quality Control

  • One step RT-PCR using eukaryotic total RNA as a template.

Storage, Shipping & Guarantee​

  • Shipped in: Gel pack.
  • Storage: -20 °C (NON Frost-Free Freezer).
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Current Promotions


AgaPure™ Agarose Standard: 169€ (500g) Learn more Here


BrightMAX™ DNA Ladders from 39 € (50 µg) Learn more Here


CANFAST™ Transfection Reagent: 99 € (1.5 mL) Learn more Here


UltraPure Water, DNASE/RNASE – Free from 34€ (1 L) Learn more Here


qMAXSen™ One-Step RT-qPCR Kits from 466 € (500 rxn) Learn more Here


XTT Cell Proliferation Assay Kit from 79 € (500 assays) Learn more Here


HigherPurity™ Blood Genomic DNA Extraction Kit: 49 € (50 rxn) Learn more Here


HigherPurity™ DNA Purification SPRI Magnetic Beads: 249€ (25 mL) Learn more Here


Horse-Power™ Green-Taq DNA Polymerase: 29 € (100 rxn) Learn more Here


Horse-Power™ Taq DNA Polymerase (500 U) from 39€ Learn more Here


Stool Sample Collection and Stabilization Kit: 319 € (100 units) Learn more Here


IPTG: 49 € (5 gr) Learn more Here


PRImeZOL™ Reagent: 79 € (100 mL) Learn more Here


Proteinase K (Lyophilized): 79 € (100 mg) Learn more Here


qMAXSen™ qPCR MasterMixes from 109 € (250 rxn) Learn more Here


Saliva Sample Collection Kit: 733 € (100 units) Learn more Here


T4 DNA Ligase: 39 € (300 U) Learn more Here


TruePure™ dNTP Mix, 1mL from 29€ Learn more Here


Resazurin Cell Viability Assay from 79 € (2,500 assays) Learn more Here


X-Gal: 59 € (1 gr) Learn more Here

Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

  • slidebg1

    Related products

    Horse-Power™ Taq DNA Polymerase

    Horse-Power™ Taq DNA Polymerase is a pure, versatile and thermostable recombinant enzyme produced in an E. coli strain, which carries the cloned pol gene from Thermus aquaticus. The enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but...


    Learn more
  • slidebg1

    Related products

    qMAXSen™ One-Step Green RT-qPCR Kit (High Rox™)

    qMAXSen™ One-Step Green RT-qPCR Kit (High Rox™) offers greater specificity, sensitivity, and yields in cDNA Synthesis and qPCR in a single tube. The kit includes a qPCR MasterMix supplied in a 2x concentration to perform Real-Time PCR using a proprietary green dye. The qPCR...


    Learn more
  • slidebg1

    Related products

    qMAXSen™ One-Step Probe RT-qPCR Kit (High Rox™)

    qMAXSen™ One-Step Probe RT-qPCR Kit (High Rox™) offers greater specificity, sensitivity, and yields in cDNA Synthesis and qPCR in a single tube. The kit includes a qPCR MasterMix supplied in a 2x concentration to perform Real-Time PCR. The qPCR MasterMix contains all the...


    Learn more
  • slidebg1

    Related products

    First Strand cDNA Synthesis Kit

    First-Strand cDNA Synthesis Kit is an Efficient & Sensitive  System for First strand cDNA Synthesis from RNA templates. It includes s all the necessary components to synthesize first-strand cDNA, except the template RNA (total RNA or mRNA). First-Strand cDNA Synthesis...


    Learn more