pOnebyOne™ Multicistronic Vector (EGFP-2A-2A) is an extremely efficient, accurate and flexible Mammalian Expression vector that allows co-expression of two Genes of Interest along with Green Fluorescent Protein (GFP) as reporter gene, driven by Human Cytomegalovirus early promoter. It is based in 2A sequence from Thosea asigna Virus (TaV) which is flanked by two Multiple Cloning […]
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GreenSafe DNA Gel Stain replace toxic Ethidium Bromide (EtBr) for the visualization of doublestranded DNA, single-stranded DNA, and RNA in Agarose and Polyacrylamide Gel Electrophoresis.
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One-Step RT PCR Kit is designed for a Highly Efficient cDNA Synthesis and PCR in a single tube. The kit includes a PCR MasterMix supplied in a 2x concentration to perform standard PCR. MasterMix contains all the reagents needed for running PCR reactions (except PCR primers and template). In addition, a separate RT Mix composed by a balanced […]
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Buccal Swab Collection & Stabilization Kit provides an efficient, safe and rapid all-in-one procedure for the collection, stabilization and transportation of buccal swab sample at ambient temperatures.
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PickMutant™ Error-Prone PCR Kit is based on the protocol of Cadwell and Joyce (1992). epPCR takes advantage of the inherently low fidelity of Taq DNA Polymerase (with errors biased toward AT to GC changes), which may be further decreased by the addition of Mn2+, increasing the Mg2+ concentration, and using unequal dNTP concentrations. The rate […]
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PickMutant™ DNA-Shuffling Kit is based on the method Developed by Stemmer. Stemmer introduced DNA shuffling, the first homologous recombination method, in 1994. DNA shuffling involves the digestion of a gene by DNase I into random fragments, and the reassembly of those fragments into a full-length gene by primerless PCR: the fragments prime on each other […]
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PCR is routinely performed with standard Polymerases, yielding a final PCR product with few errors. Although Polymerases vary in fidelity, the inherent Mutation rate of PCR is insufficient to generate the high frequency of mutations necessary to generate a mutant library. The addition of nucleotide analogues to the PCR reaction reduces Polymerase fidelity increased substitution frequency in the […]
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BL21 (DE3) Competent Cells are Chemically Competent Escherichia coli Cells used for Protein Expression of T7 RNA Polymerase-based Systems. These cells are resistant to the lytic bacteriophages T1 and T5. The BL21 (DE3) strain is a derivative of E. coli B. It is deficient in both lon and ompT proteases resulting in superior isolation of intact […]
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qMAXSen™ One-Step Probe RT-qPCR Kit (High Rox™) offers greater specificity, sensitivity, and yields in cDNA Synthesis and qPCR in a single tube. The kit includes a qPCR MasterMix supplied in a 2x concentration to perform Real-Time PCR. The qPCR MasterMix contains all the reagent (except PCR Primers, Probe and Template) needed for running PCR reactions. The Mix is […]
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qMAXSen™ One-Step Probe RT-qPCR Kit (Low Rox™) offers greater specificity, sensitivity, and yields in cDNA Synthesis and qPCR in a single tube. The kit includes a qPCR MasterMix supplied in a 2x concentration to perform Real-Time PCR. The qPCR MasterMix contains all the reagent (except PCR Primers, Probe and Template) needed for running PCR reactions. The Mix is compatible with […]
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