pSpark® I DNA Cloning Kit

pSpark® I DNA Cloning Kit

Product Overview:

pSpark® I is a highly efficient, accurate and easy-to-use DNA cloning System based on a novel breakthrough technology to generate blunt vectors with a high blunt cloning efficiency. The vector is prepared by digestion of pSpark® at the EcoRV site before treating both ends to prevent vector self-ligation. The end treatment is supported by a proprietary […]

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pSpark® II DNA Cloning Kit

pSpark® II DNA Cloning Kit

Product Overview:

pSpark® II is a highly efficient, accurate and easy-to-use DNA Cloning system based on a breakthrough technology for cloning blunt ended DNA generated by PCR with a proofreading or high fidelity DNA polymerases. The vector is prepared by digestion of pSpark® II at the EcoRV site before treating both ends to prevent vector self-ligation. The […]

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pSpark® III DNA Cloning Kit

pSpark® III DNA Cloning Kit

Product Overview:

pSpark® III is a highly efficient, accurate and easy-to-use DNA Cloning System that combines Ampicillin and Kanamycin resistance. Ideal for cloning PCR products amplified from any plasmid vector without the need to gel-purify bands to eliminate the background due to the template vector used for PCR.

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pSpark® IV DNA Cloning Kit

pSpark® IV DNA Cloning Kit

Product Overview:

pSpark® IV is a highly efficient, accurate and easy-to-use DNA cloning system that exploit its very low background feature for the expression of toxic genes under transcription-free conditions. In this vector, the lac promoter has been eliminated and therefore blue/white screening is not allowed (alpha-peptide coding region remains and you can find blue colony). The […]

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pSpark® V DNA Cloning Kit

pSpark® V DNA Cloning Kit

Product Overview:

pSpark® V is a highly efficient, accurate and easy-to-use DNA cloning system developed with low copy number, as a help for cloning of inserts with the highers kb. This low copy variant is also transcription-free, for the most demanding cloning tasks. In this vector, the lac promoter has been eliminated and therefore blue/white screening is […]

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pSpark® Done DNA Cloning Kit

pSpark® Done DNA Cloning Kit

Product Overview:

pSpark® Done is a highly efficient, accurate and easy-to-use DNA Cloning system designed for cloning of blunt ended DNA with very high efficiency. The MCS of the pSpark® Done vector incorporates sequences on either side of the insert that are recognized by the restriction enzymes NotI and EcoRI. This allows the insert DNA to be […]

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pSpark® TA DNA Cloning Kit

pSpark® TA DNA Cloning Kit

Product Overview:

pSpark® TA is efficient, stable and easy-to-use DNA cloning vector based on an optimized TA technology for cloning single 3 ́-adenine overhanging DNA. The vectors are prepared by digestion of pSpark® TA at EcoRV site and the subsequent addition of a single thymidine at each 3 ́- end to allow cloning Taq DNA Polymerase amplified […]

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pSpark® TA Done DNA Cloning Kit

pSpark® TA Done DNA Cloning Kit

Product Overview:

pSpark® TA Done is efficient, stable and easy-to-use DNA cloning vector based on an improved TA technology that offers all of the advantages of pSpark® TA with the added convenience of recognition sites for EcoRI and NotI flanking the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction […]

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CVX5α™ Chemically Competent Cells

CVX5α™ Chemically Competent Cells

Product Overview:

CVX5α™ Chemically Competent Cells is a versatile, convenient and cost-effective solution for routine subcloning procedures or any application where the starting DNA is not limiting. It is calcium chloride-treated to facilitate attachment of the plasmid DNA to the competent cell membrane. CVX5α™ Genotype:  F – , gyrA96, recA1, endA1, thi1, hsdR17 (rK – mK +), deoR, […]

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pMBL™ T-Vector DNA Cloning Kit

pMBL™ T-Vector DNA Cloning Kit

Product Overview:

pMBL™ T-Vector DNA Cloning Kit is an efficient, convenient and fast system for the cloning of PCR products. The vector is prepared by cutting pMBL™ T-Vector with EcoRV and adding a 3´ terminal thymidine to both ends. These single 3´-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product […]

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