pColiExpress™ II Cloning & Expression Kit
For a highly Efficient, Fast & Versatile Protein Expression (T4 DNA Ligase based) in E. coli from PCR fragment cloned
Product Overview:
pColiExpress™ II Cloning & Expression Kit is a DNA Cloning System for Protein Expression in E.coli. pColiExpress™ technology allows efficiently cloning DNA fragments and the quick production of a large quantity of any desired Protein.
pColiExpress™ II contains all elements required for expression of large quantity of any desired protein by T7 RNA Polymerase Inducible System and a His6 tag at COOH end that allow the protein purification. The vector has also an f1 origin of replication, an ampicillin resistance cassette, and a pBR322 origin of replication.
Quote requestAvailability: In stock! Ready-to-ship immediately
Catalog Numbers, Sizes & Prices
| Cat. No. | Size | Price |
| BE006 | 20 rxn | 135 € |
| BE007 | 20 rxn-Plus | 179 € |
Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.
Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.
Advantages & Features
- Ready-to-use vectors.
- Highly efficient cloning system.
- Special design that allows the cell to house larger numbers of copies than other plasmids with ori pBR322.
- Linearized vector: ready for ligation with your PCR amplified with the recommended primers.
- Minimum background: lower than 1%.
- Time-saving protocol: avoids any step required after PCR.
- Higher protein expression than other suppliers.
- Cost avoidance: avoids the use of expensive phosphorylated primers.
- Versatility: cloning of PCR fragments amplified with any type of DNA Polymerase.
- Risk-free: product covered by our Quality 100% Guarantee.
Specifications
- T7 Promoter: 4605-4621
- Lac O: 4627-4645
- T7 transcription start: 4621
- His Tag: 4710-4727
- T7 terminator: 4749-4796
- LacIq: 96-1175
- Ori pBR322: 2970-2356
- Ampicillin resistance gene (ORF): 3133-3990
- F1 Origin: 4562-4115
Includes
Includes for 20 rxn:
– 20 µL pColiExpress™ linearized vector (50 ng/µL)
– 200 µL T4 DNA Ligase Buffer (5x)
– 20 µL T4 DNA Ligase (5 U/µL)
– 10 µL Control Insert DNA (30 ng/µL)
– 5 µL pColiExpress™ Control (50 ng/µL)
Plus Version Includes for 20 rxn
– 20 µL pColiExpress™ linearized vector (50 ng/µL)
– 200 µL T4 DNA Ligase Buffer (5x)
– 20 µL T4 DNA Ligase (5 U/µL)
– 10 µL Control Insert DNA (30 ng/µL)
– 5 µL pColiExpress™ Control (50 ng/µL)
– 20 µL EcoRV (10 U/µL)
– 20 µL Hind III (10 U/µL)
– 1.5 mL Buffer C (10x)
Download documentation
Data SheetApplications
- Cloning of PCR fragments for subsequent expression of proteins in E. coli.
- Expression of Proteins under the control of the T7 promoter.
- Protein expression in BL21 (DE3) or BL21 (DE3) (pLys).
Tables, Figures & Protocols
Quality Control
- Efficiency of ligation with the insert Control >1,000 cfu with 1:5 relationship vector:insert using CVX5α™ Competent Cells with > 107 cfu/µg.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
- Shelf Life: 24 months from the date of purchase, if it is properly stored.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
