pColiExpress™ III LIC Cloning & Expression Kit

pColiExpress™ III LIC Cloning & Expression Kit

For a highly Efficient & Versatile Protein Expression (LIC technology based) in E. coli from PCR fragment cloned

Product Overview:

pColiExpress™ III LIC Cloning & Expression Kit is a highly efficient, versatile and fast system of DNA cloning vectors for protein expression in E. coli. All family systems are based in directional ligase-independent-cloning (LIC) technology, a rapid procedure that provides high-cloning efficiencies and the fast production of a large quantity of any desired protein.

It contains all elements required for periplasmic expression of any desired protein by OMPA Periplasmatic Signal peptide and T7 RNA Polymerase Inducible System.  It includes a His6 tag at COOH end of the protein that allows the purification. The vector also has an f1 origin of replication, an Ampicillin resistance cassette, and the pBR322 origin of replication.

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Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
BE010-S 10 rxn 89 €  69€
BE010 20 rxn 139 €

Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Ready-to-use vectors.
  • Highly efficient cloning system.
  • Special design that allows the cell to house larger numbers of copies than other plasmids with ori pBR322.
  • Linearized vector: ready for ligation with your PCR amplified with the recommended primers.
  • Minimum background: lower than 1%.
  • Time-saving protocol: avoids any step required after PCR.
  • Higher protein expression than other suppliers.
  • Cost avoidance: avoids the use of expensive phosphorylated primers.
  • Versatility: cloning of PCR fragments amplified with any type of DNA Polymerase.
  • Risk-free: product covered by our Quality 100% Guarantee.


  • T7 Promoter: 4605-4621
  • LacO: 4627-4645
  • T7 transcription start: 4621
  • Signal Peptide OmpA: 4696-4777
  • His6 Tag: 4786-4803
  • T7 terminator: 4825-4872
  • LacIq: 96-1175
  • Ori pBR322: 2970-2356
  • AMP R (Ampicillin resistance gene): 3133-3990
  • f1 Origin: 4562-4115


Includes for 20 rxn:

– 20 µL pColiExpress™ linearized vector (50 ng/µL)

– 50 µL LIC Enzyme Buffer (10x)

– 40 µL LIC Enzyme (10 U/µL)

– 10 µL Insert Control DNA (30 ng/µL)

– 5 µL pColiExpress™ Control – Uncut vector (50 ng/µL)


Download documentation

Data Sheet


  • Cloning of PCR fragments for subsequent expression of proteins in E. coli.
  • Cloning of PCR fragments with Glue Enzyme.
  • Expression of proteins under the control of the T7 promoter.
  • Protein expression in BL21 (DE3) or BL21 (DE3) (pLys).

Tables, Figures & Protocols

pColiExpress III

Quality Control

  • Efficiency of ligation with the insert Control >1,000 cfu with 1:5 relationship vector:insert using CVX5α™ Competent Cells with > 107 cfu/µg.

Storage, Shipping & Guarantee​

  • Shipped in: Gel pack.
  • Storage: -20 ºC (NON Frost-Free Freezer).
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Current Promotions

Citations Reward Program, get 50€ Amazon gift card Learn more Here

Lab Start-Up Program for new labs/companies Learn more Here

Bulk & Volume discounts for larger quantities Learn more Here

Frequently asked questions

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Customers Review

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