PickMutant™ Error Prone PCR Kit

PickMutant™ Error Prone PCR Kit

For a Reliable, Robust & highly Efficient Random Mutagenesis by Error-Prone PCR

Product Overview:

PickMutant™ Error-Prone PCR Kit is based on the protocol of Cadwell and Joyce (1992). epPCR takes  advantage of the inherently low fidelity of Taq DNA Polymerase (with errors biased toward AT to GC changes), which may be further decreased by the addition of Mn2+, increasing the Mg2+ concentration, and using unequal dNTP concentrations. The rate of Mutagenesis achieved by error-prone PCR is in the range of 0.6-2.0 %.

Quote request

Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No.SizePrice
MT04030 rxn224 €

 

We also offer this Product with different specifications or size (Custom Solutions), in higher amounts (Bulk Inquiries) or under OEM Basis (OEM Partnerships).

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Highly Efficient.
  • Reliable.
  • Robust.

Includes

Includes for 30 rxn:

– 50 μL Taq Polymerase (5 U/μL)

– 200 μL 10x ep-PCR Buffer

– 80 μL ep-dNTP Mix (unbalanced dNTP ratio)

– 200 μL MnCl2 Solution (25mM)

– 2 x 1.5 mL Water  (PCR Grade)

Download documentation

Data Sheet

Applications

  • Generation of a library of mutants.
  • In vitro Molecular evolution.

Tables, Figures & Protocols

Error-Prone PCR Kit

Quality Control

  • Tested using the insert control DNA provided.

Storage, Shipping & Guarantee​

  • Shipped in: Gel pack.
  • Storage: -20 ºC (NON Frost-Free Freezer) and Avoid freeze/thaw cycles.
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Citations

Wong, T. S., & Tee, K. L. (2020). Gene Mutagenesis. In A Practical Guide to Protein Engineering (pp. 121-148). Springer, Cham.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Current Promotions


AgaPure™ Agarose Standard: 169€ (500g) Learn more Here


BrightMAX™ DNA Ladders from 39 € (50 µg) Learn more Here


CANFAST™ Transfection Reagent: 99 € (1.5 mL) Learn more Here


UltraPure Water, DNASE/RNASE – Free from 34€ (1 L) Learn more Here


qMAXSen™ One-Step RT-qPCR Kits from 466 € (500 rxn) Learn more Here


XTT Cell Proliferation Assay Kit from 79 € (500 assays) Learn more Here


HigherPurity™ Blood Genomic DNA Extraction Kit: 49 € (50 rxn) Learn more Here


HigherPurity™ DNA Purification SPRI Magnetic Beads: 249€ (25 mL) Learn more Here


Horse-Power™ Green-Taq DNA Polymerase: 29 € (100 rxn) Learn more Here


Horse-Power™ Taq DNA Polymerase (500 U) from 39€ Learn more Here


Stool Sample Collection and Stabilization Kit: 319 € (100 units) Learn more Here


IPTG: 49 € (5 gr) Learn more Here


PRImeZOL™ Reagent: 79 € (100 mL) Learn more Here


Proteinase K (Lyophilized): 79 € (100 mg) Learn more Here


qMAXSen™ qPCR MasterMixes from 109 € (250 rxn) Learn more Here


Saliva Sample Collection Kit: 733 € (100 units) Learn more Here


T4 DNA Ligase: 39 € (300 U) Learn more Here


TruePure™ dNTP Mix, 1mL from 29€ Learn more Here


Resazurin Cell Viability Assay from 79 € (2,500 assays) Learn more Here


X-Gal: 59 € (1 gr) Learn more Here

Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

  • slidebg1

    Related products

    PickMutant™ Site-directed Mutagenesis Kit

    PickMutant™ is a reliable, robust and highly efficient PCR-based Mutagenesis Kit. Extremely easy-to-use, the kit allows creating single or multiple point mutations, deletions or insertions using a rapid and easy protocol. All these mutation could be obtained by PCR using...


    Learn more
  • slidebg1

    Related products

    PickMutant™ Random Mutagenesis Kit

    PCR is routinely performed with standard Polymerases, yielding a final PCR product with few errors. Although Polymerases vary in fidelity, the inherent Mutation rate of PCR is insufficient to generate the high frequency of mutations necessary to generate a mutant library. The addition...


    Learn more
  • slidebg1

    Related products

    PickMutant™ DNA Shuffling Kit

    PickMutant™ DNA-Shuffling Kit  is based on the method Developed by Stemmer. Stemmer introduced DNA shuffling, the first homologous recombination method, in 1994. DNA shuffling involves the digestion of a gene by DNase I into random fragments, and the reassembly of...


    Learn more