Poly(A) Polymerase

Poly(A) Polymerase

High Quality & Purity Enzyme manufactured from Recombinant source

Product Overview:

Poly(A) Polymerase I from Escherichia coli, is a very active recombinant enzyme produced by a overproducer strain of E.coli. The enzyme adds a long tail of adenine nucleotides at the 3’ end of messenger RNA using ATP as donor.

 

GMP-grade is also available. Learn more.

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Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Concentration Price
EZ0037 100 U 5 U/µL 64 €

 

We  also offer this Product with different specifications (GMP-grade is also available) or size (Custom Solutions), in higher amounts (Bulk Inquiries) or under OEM Basis (OEM Partnerships).

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • High-Purity: ≥ 95% determined by SDS.
  • Recombinant: isolated from E.coli.
  • Extensivelly quality controlled: Endo/exonucleases, non-specific DNAse and RNAses free, Purity and Promoter Specificity.
  • GMP-grade available.

Specifications

Protein information
Predicted MW: 54 kDa
Purity: ≥ 95% as determined by SDS-polyacrylamide gels with Coomassie® blue staining.
Buffer Storage: 1X Reaction Buffer: 20 mM Tris-HCl, 300 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.1 % Triton®X-100, 50% Glycerol, (pH 7.5 at 25°C).
Biological activity: One unit is defined as the amount of enzyme that will incorporate 1 nmol of AMP into RNA in a 20 µl volume in 10 minutes at 37°C.

 

Gene Information

Synonyms: PAP I
Official Symbol: pcnB
Specie: Escherichia coli
GenBank accession number: NC_000913 (Range 157729..159126)
NCBI Protein RefSeq: NP_414685.4
Protein Family: polyA polymerase

Includes

Includes:

– 100 U Poly(A) Polymerase, from E. coli (5 U/µL)
– Adenosine-5′-Triphosphate (ATP)
– Poly(A) Polymerase Reaction Buffer

Download documentation

Data Sheet
Brochure

Applications

  • Labeling of RNA with ATP or cordycepin.
  • Poly(A) tailing of RNA for cloning or affinity purification
  • Enhances translation of RNA transferred into eukaryotic cells.

Quality Control

Quality Tests for Research Use Only version includes: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription, etc.

Request your CoA here.

Advice

Avoid exposure to constant temperature changes.

Storage, Shipping & Guarantee​

  • Shipped in: Gel Pack.
  • Storage: -20 ºC (NON Frost-Free Freezer).

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

To be informed about our Safety Staments for GMP Products, please contact us.

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Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

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