pOnebyOne™ FREE Bicistronic Mammalian Expression Kit
Extremely Efficient, Accurate and Flexible vector to clone your genes and express your Proteins of Interest
Product Overview:
pOnebyOne™ are extremely efficient, accurate and flexible Bicistronic Mammalian Expression Kits that contains an expression cassette based in 2A sequence Breakthrough technology.
pOnebyOne™ FREE allows co-expression of two genes of interest driven by Human Cytomegalovirus early promoter. It is based in 2A sequence from Thosea asigna Virus (TaV) which is flanked by two multiple cloning sites. T2A sequence leads to the highest level of Protein Expression at the second gene position compared to IRES sequence.
The mechanism of 2A self-cleavage is a ribosome skipping process where the ribosome skips the formation of a glycil-prolyl peptide bond at the C-terminus of 2A. Stable mammalian cells could be selected by neomycin resistance.
Both genes of interest must be in frame with T2A and between them. Successful skipping results in translation of two proteins: 2A upstream protein is attached to the 2A sequence (GSGEGRGSLLTCGDVEENPG) at C-terminus while 2A downstream protein is attached to one Pro at the N-terminus.
Quote requestAvailability: In stock! Ready-to-ship immediately
Catalog Numbers, Sizes & Prices
| Cat. No. | Size | Price |
| MEF020 | 20 rxn (1 µg) | 389 € |
Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.
Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.
Advantages & Features
- Complete solution: a cloning vector to clone and produce two proteins of interest.
- Breakthrough technology: based in 2A sequence simultaneous expression of two proteins in mammalian cells (for example, a protein of interest and reporter).
- Highly efficient: tested with up to 4 kb inserts.
- Accurate: proven performance for most common cell lines.
- Flexible: allows transfection in difficult cell lines.
- Convenient: available with different resistance marker cassettes.
- Reporter checking: stoichiometric amount of your protein of interest and a reporter protein.
- Risk-free: product covered by our Quality 100% Guarantee.
Specifications
- Cytomegalovirus Promoter: 232-887
- Multiple Cloning site 1 (MCS 1): 895-967
- T2A from Thosea asigna virus: 968-1030
- Multiple Cloning site 2 (MCS 2): 1031-1065
- BGH Polyadenylation Sequence: 1082-1306
- f1 Origin: 1362-1780
- Neomycin Resistance Cassette: 1785-3288
- Neomycin Resistance Gene (ORF): 2190-2981
- pUC Origin: 3671-4341
- Ampicillin Resistance Cassette (complementary strand): 4486-5480
- Ampicillin Resistance Gene (ORF): 4489-5346
Includes
Includes:
– 20 µL pOnebyOne™ FREE (50 ng/ µL) – as Closed Circular Vector
Download documentation
Data SheetApplications
- Protein Expression of intracellular, extracellular or transmembrane higher cells in an equimolecular to a surface marker that allows quantification.
Tables, Figures & Protocols
Quality Control
- Cloning of SEAP open reading frame and testing phosphatase alkaline activity in mammalian cells.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
- Shelf Life: 12 months from the date of purchase, if it is properly stored.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
