pOnebyOne™ III – Lentiviral Bicistronic Mammalian Expression Kit
Extremely Efficient lineal vector ready-to-clone your PCR gene and express your Protein of Interest
Product Overview:
pOnebyOne™ are extremely efficient, accurate and flexible Bicistronic Mammalian Expression Kits that contains an expression cassette based in 2A sequence Breakthrough technology. The expression cassette of pOnebyOne™ III – Lentiviral incorporates the Human elongation factor 1 alpha promoter that precedes 2A sequence in frame with the truncated nerve growth factor receptor (ΔNGFR). ΔNGFR is a complete solution to select positive clones as they can be visualized by cytometry using a specific antibody labelled with FITC or using a similar method and also, they can be enriched from negative clones with magnetic beads bearing an anti-ΔNGFR antibody.
Quote requestAvailability: In stock! Ready-to-ship immediately
Catalog Numbers, Sizes & Prices
| Cat. No. | Size | Price |
| ME0019-S | 10 rxn | 323 € |
| ME0019 | 20 rxn | 539 € |
Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.
Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.
Advantages & Features
- Complete solution: a directional cloning vector to clone and produce a protein of interest.
- Breakthrough technology: based in 2A sequence simultaneous expression of two proteins in mammalian cells (a protein of interest and reporter).
- Highly efficient: cloning system tested with up to 4 kb inserts.
- Time-saving cloning process: linearized vector ready-to-mix with your PCR fragment.
- Easy-to-use: facilitates the selection of positive cells expressing the recombinant gene of interest.
- Cost avoidance: avoids the use of restriction enzymes.
- Accurate: proven performance for most common cell lines.
- Flexible: allows transfection in difficult cell lines.
- Really low experimental background: lower than 1%.
- Convenient: available with different resistance marker cassettes.
- Directional cloning: of PCR with the gene of interest.
- Reporter checking: stoichiometric amount of your protein of interest and a reporter protein.
- Risk-free: product covered by our Quality 100% Guarantee.
Includes
Includes for 20 rxn:
– 20 µL Linearized vector (50 ng/µL)
– 40 µL Glue Enzyme (10 u/µL)
– 50 µL Glue Enzyme Buffer (10x)
– 10 µL Insert Control (30 ng/µL)
– 5 µL pOnebyOne™ Control (50 ng/µL)
Download documentation
Data SheetBrochureApplications
- Highest generation Lentiviral transfer vector to transform replicating and non-replicating cells, including stem and neuronal cells.
- Vector of choice for short-interfering RNA (siRNA) delivery and for gene therapy.
Tables, Figures & Protocols
Figure: 2A sequence based vector.
Quality Control
- Cloning of SEAP open reading frame and testing phosphatase alkaline activity in mammalian cells.
Advice
Note: pOnebyOne™ Lentiviral expression vectors might be unstable in some hematopoietic lines.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
- Shelf Life: 24 months from the date of purchase, if it is properly stored.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
