pOnebyOne™ III – Neo Bicistronic Mammalian Expression Kit

pOnebyOne™ III – Neo Bicistronic Mammalian Expression Kit

Extremely Efficient lineal vector ready-to-clone your PCR gene and express your Protein of Interest

Product Overview:

pOnebyOne™ are extremely efficient, accurate and flexible Bicistronic Mammalian Expression Kits that contains an expression cassette based in 2A sequence Breakthrough technologyThe expression cassette of pOnebyOne™ III (with Neomycin resistance) incorporates the Human elongation factor 1 alpha promoter that precedes 2A sequence, in frame with the truncated nerve growth factor receptor (ΔNGFR). ΔNGFR is a complete solution to select positive clones. It can be visualized by cytometry using a specific antibody labelled with FITC or a similar method, and also, it can be enriched from negative clones with magnetic beads bearing an anti-ΔNGFR antibody.

Quote request

Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
ME003-N-S 10 rxn 229 €
ME003-N 20 rxn 389 €

Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Complete solution: a directional cloning vector to clone and produce a protein of interest.
  • Breakthrough technology: based in 2A sequence simultaneous expression of two proteins in mammalian cells (a protein of interest and reporter).
  • Highly efficient: cloning system tested with up to 4 kb inserts.
  • Time-saving cloning process: linearized vector ready-to-mix with your PCR fragment.
  • Easy-to-use: facilitates the selection of positive cells expressing the recombinant gene of interest.
  • Cost avoidance: avoids the use of restriction enzymes.
  • Accurate: proven performance for most common cell lines.
  • Flexible: allows transfection in difficult cell lines.
  • Really low experimental background: lower than 1%.
  • Convenient: available with different resistance marker cassettes.
  • Directional cloning: of PCR with the gene of interest.
  • Reporter checking: stoichiometric amount of your protein of interest and a reporter protein.
  • Risk-free: product covered by our Quality 100% Guarantee.


  • Elongation factor 1α promoter:  19-1220.
  • Cloning site:  1225.
  • 2A from equine rhinitis A virus:  1229-1291.
  • ΔNGFR: 1298-2133.
  • BGH polyadenylation sequence:  2168-2392.
  • f1 origin:  2448-2866.
  • Neomycin resistance cassette:  2866-4374.
  • Neomycin resistance gene (ORF):  3276-4067.
  • pUC origin:  4757-5427.
  • Ampicillin resistance gene (ORF) (complementary strand):  5575-6432.


 Includes for 20 rxn:

– 20 µL Linearized vector (50 ng/µL)

– 40 µL Glue Enzyme (10 u/µL)

– 50 µL Glue Enzyme Buffer (10x)

– 10 µL Insert Control (30 ng/µL)

– 5 µL pOnebyOne™ Control (50 ng/µL)

Download documentation

Data Sheet


  • Protein Expression of intracellular, extracellular or transmembrane higher cells in an equimolecular to a surface marker that allows quantification.

Tables, Figures & Protocols

pOnebyOne III


                     Figure: 2A sequence based vector.

2A sequence based vector

Quality Control

  • Cloning of SEAP open reading frame and testing phosphatase alkaline activity in mammalian cells.

Storage, Shipping & Guarantee​

  • Shipped in: Gel pack.
  • Storage: -20 ºC (NON Frost-Free Freezer).
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Current Promotions

Citations Reward Program, get 50€ Amazon gift card Learn more Here

Lab Start-Up Program for new labs/companies Learn more Here

Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

  • slidebg1

    Related products

    CANFAST™ Transfection Reagent

    CANFAST™ is a Highly Efficient, Ready-to-& Non-Toxic new generation cationic Polymer. It has important features as DNA condensation and endosomal release, which improves gene transfection...

    Learn more
  • slidebg1

    Related products

    FastCONTROL™ p2V-LacZ/ ΔNGFR-I – Dual Reporter

    FastCONTROL™ Dual Reporters are fast, convenient and flexible target cell transfection control ideal for high co-expression of two reporter genes drive for ubiquitous, strong and constitutive promoters. FastCONTROL™ p2V-LacZ/ ΔNGFR-I Dual Reporter vector contains cytomegalovirus promoter (Pcmv) to drive the expression...

    Learn more
  • slidebg1

    Related products

    FastPANGEA™ Long PCR DNA Polymerase

    FastPANGEA™ Long PCR DNA Polymerase is an accurate and robust enzyme that combines Horse-Power™ Taq DNA Polymerase and a DNA proofreading Polymerase with 3’ to 5’ exonuclease activity that is optimized for PCR amplification of very long DNA templates (long...

    Learn more
  • slidebg1

    Related products

    pASSEMBLE™ Lentiviral Packaging System

    pASSEMBLE™ Lentiviral Packaging System includes an optimized mix of three vectors with sequences of gag, pol and rev genes from human immunodeficiency virus (HIV-1) and the envelope gene from vesicular stomatitis virus (VSV-G). VSV-G envelope confers a wide range of tropism...

    Learn more
  • slidebg1

    Related products

    WideUSE™ Plasmid Purification Kit

    WideUse™ Plasmid Purification Kit offers a reliable, convenient and rapid method for routinary isolation of high quality plasmid preparations in mini format. The kit uses HigherPurity™ breakthrough technology based in DNA ability to bind silica in the presence of high...

    Learn more