pOnebyOne™ III – Puro Bicistronic Mammalian Expression Kit
Extremely Efficient lineal vector ready-to-clone your PCR gene and express your Protein of Interest
pOnebyOne™ are extremely efficient, accurate and flexible Bicistronic Mammalian Expression Kits that contains an expression cassette based in 2A sequence Breakthrough technology. The expression cassette of pOnebyOne™ III (with Puromycin resistance) incorporates the Human elongation factor 1 alpha promoter that precedes 2A sequence, in frame with the truncated nerve growth factor receptor (ΔNGFR). ΔNGFR is a complete solution to select positive clones. It can be visualized by cytometry using a specific antibody labelled with FITC or a similar method, and also, it can be enriched from negative clones with magnetic beads bearing an anti-ΔNGFR antibody.Quote request
Availability: In stock! Ready-to-ship immediately
Catalog Numbers, Sizes & Prices
|ME003-P-S||10 rxn||229 €|
|ME003-P||20 rxn||389 €|
Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.
Advantages & Features
- Complete solution: a directional cloning vector to clone and produce a protein of interest.
- Breakthrough technology: based in 2A sequence simultaneous expression of two proteins in mammalian cells (a protein of interest and reporter).
- Highly efficient: cloning system tested with up to 4 kb inserts.
- Time-saving cloning process: linearized vector ready-to-mix with your PCR fragment.
- Easy-to-use: facilitates the selection of positive cells expressing the recombinant gene of interest.
- Cost avoidance: avoids the use of restriction enzymes.
- Accurate: proven performance for most common cell lines.
- Flexible: allows transfection in difficult cell lines.
- Really low experimental background: lower than 1%.
- Convenient: available with different resistance marker cassettes.
- Directional cloning: of PCR with the gene of interest.
- Reporter checking: stoichiometric amount of your protein of interest and a reporter protein.
- Risk-free: product covered by our Quality 100% Guarantee.
- Elongation factor 1α promoter: 19-1220.
- Cloning site: 1225.
- 2A from equine rhinitis A virus: 1229-1291.
- ΔNGFR: 1298-2133.
- BGH polyadenylation sequence: 2190-2414.
- f1 origin: 2470-2888.
- Puromycin resistance gene (ORF): 3263-3859.
- pUC origin: 4447-5117.
- Ampicillin resistance gene (ORF) (complementary strand): 5265-6122.
Includes for 20 rxn:
– 20 µL Linearized vector (50 ng/µL)
– 40 µL Glue Enzyme (10 u/µL)
– 50 µL Glue Enzyme Buffer (10x)
– 10 µL Insert Control (30 ng/µL)
– 5 µL pOnebyOne™ Control (50 ng/µL)
- Protein Expression of intracellular, extracellular or transmembrane higher cells in an equimolecular to a surface marker that allows quantification.
Tables, Figures & Protocols
- Cloning of SEAP open reading frame and testing phosphatase alkaline activity in mammalian cells.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
- Shelf Life: 24 months from the date of purchase, if it is properly stored.
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.