PRImeDETECT™ Listeria monocytogenes Detection Kit

PRImeDETECT™ Listeria monocytogenes Detection Kit

For a Fast & Reliable Amplification and Detection of specific DNA fragments from Listeria monocytogenes by the real-time PCR

Product Overview:

PRImeDETECT™ Listeria monocytogenes Detection Kit is an ideal tool for a fast and reliable Amplification and Detection of specific DNA fragments from Listeria monocytogenes by the real-time PCR method.

The Kit includes all reagents required in a comfortable ready-to-use Multiplex PCR MasterMix. The optimized MasterMix contains a Buffer, dNTPs, Hot-start DNA Polymerase, DNA-free water, MgCl2 and an Internal Amplification Control (IAC) whose detection indicates the absence of PCR inhibitors.

Primers and Probes for the amplification of IAC as well as for the amplification of the target gene are included in the MasterMix. The Probe for the detection of target gene is labelled with the FAM, whereas the probe for the detection of IAC is labelled with the HEX fluorochrome.

In addition, the kit includes both Positive Control and Negative Control. The Positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNAready Lysis Buffer to extract the DNA from the sample prior to PCR detection.

Quote request

Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
FP0015 48 rxn 209 €
FP0016 96 rxn 319 €

Would you like to test a Free Sample? Please, inquiry at Free Sample Request.

We also offer this Product with different specifications or size (Custom Solutions), in higher amounts (Bulk Inquiries) or under OEM Basis (OEM Partnerships).

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Easy and Ready-to-use Kit.
  • Fast: results in less than 24 hours with minimal handling.
  • Reliable and highly specific Results.
  • High Quality: manufactured according strict quality controls in every manufacturing step to guarantee the highest reproducibility.
  • Customizable Multiplex Solutions: according to your requirements, to measure up to 5 Pathogens of your choice at the same time in 1 kit. Ask us how now.
  • Risk-free: product covered by Canvax™ Guarantee. 

Specifications

  • Amplification limit: 10 UG per reaction (100%), 2 UG per reaction (40%)
  • Quantification limit:  20 UG per reaction (100%).
  • Quantification Dynamic range: 6 logs
  • Target: Listeria monocytogenes
  • Inclusivity: Positive in 48 reference strains of L. monocytogenes (serovars 1 to 7) from different collections.
  • Exclusivity:  100% Tested with 94 nontarget strains composed of 51 strains of Listeria nomonocytogenes and 43 strains of non-Listeria.
  • Detection: Probe labelled with fluorescent dyes Listeria monocytogenes: FAM-BHQ1a; IAC: HEXBHQ1.
  • Thermal Cycler: Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers.

Includes

Includes for 48 rxn:

– 1 x PCR MasterMix

– 1 x DNAready Lysis Buffer

– 1 x PCR Positive Control

– 1 x PCR Negative Control

Download documentation

Data Sheet
MSDS
Brochure

Quality Control

  • Strict quality controls in every manufacturing step to guarantee the highest quality and reproducibility.

Storage, Shipping & Guarantee​

  • Shipment at Ambient Temperature.
  • Storage: -20 °C upon receipt. Avoid prolonged exposure to light. If stored correctly the kit will retain full activity for 12 months. The kit can be stored at 4°C for 1 month.
  • Shelf Life: 12 months from the date of purchase, if it is properly stored.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

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