PRImeDETECT™ Salmonella Plus Detection Kit
For a Fast & Reliable Amplification and Detection of specific DNA fragments from Salmonella spp and/or Listeria monocytogenes by the real-time PCR
PRImeDETECT™ Salmonella Plus Detection Kit (Salmonella spp & Listeria monocytogenes) is an ideal tool for a fast and reliable Amplification and Detection of specific DNA fragments from Salmonella spp and/or Listeria monocytogenes by the real-time PCR method (multiplex PCR).
The Kit includes all reagents required in a comfortable ready-to-use Multiplex PCR MasterMix. The optimized MasterMix contains a Buffer, dNTPs, Hot-start DNA Polymerase, DNA-free water, MgCl2 and an Internal Amplification Control (IAC) whose detection indicates the absence of PCR inhibitors.
Primers and Probes for the amplification of IAC as well as for the amplification of the target gene are included in the MasterMix. The Probe for the detection of target gene is labelled with the FAM (Listeria) and HEX fluorochrome (Salmonella), whereas the probe for the detection of IAC is labelled with the CY5 fluorochrome.
In addition, the kit includes both Positive Control and Negative Control. The Positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNAready Lysis Buffer to extract the DNA from the sample prior to PCR Detection.Quote request
Availability: In stock! Ready-to-ship immediately
Catalog Numbers, Sizes & Prices
|FP0010||48 rxn||269 €|
|FP0011||96 rxn||466 €|
Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.
Advantages & Features
- Easy and Ready-to-use Kit.
- Fast: results in less than 24 hours with minimal handling.
- Reliable and highly specific Results.
- Amplification limit: 10 UG per reaction (100%).
- Quantification limit: 20 UG per reaction (100%).
- Quantification Dynamic range: 6 logs
- Simultaneously detect and differentiate Salmonella spp. and Listeria monocytogenes in the same tube
- Inclusivity: Salmonella: Positive in 30 reference strains of food origin from the Spanish Type Culture Collection (CECT). Listeria: Positive in 48 reference strains of L. monocytogenes (serovars 1 to 7) from different collections.
- Exclusivity: Salmonella: Tested with 29 nontarget strains. Listeria: 100% Tested with 94 nontarget strains composed of 51 strains of Listeria nomonocytogenes and 43 strains of non-Listeria.
- Detection: probe labelled with fluorescent dyes– Salmonella: HEX-BHQ1; Listeria: FAM-BHQ1a; IAC:CY5-BHQ2.
- Thermal cycler: Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers.
Includes for 48 rxn:
– 1 x Multiplex PCR MasterMix
– 1 x DNAready Lysis Buffer
– 1 x PCR Positive Control
– 1 x PCR Negative Control
Storage, Shipping & Guarantee
- Shipment at Ambient Temperature.
- Storage: -20 °C upon receipt. Avoid prolonged exposure to light. If stored correctly the kit will retain full activity for 12 months. The kit can be stored at 4°C for 1 month.
- Shelf Life: 12 months from the date of purchase, if it is properly stored.
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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