Proteinase K Solution

Proteinase K Solution

Molecular Biology Grade, 20 mg/mL

Product Overview:

Proteinase K Solution is a highly active serine protease that exhibits broad cleavage specificity.  Isolated from Tritirachium album is used for protease digestion during DNA and RNA preparation. With a molecular weight 28.900 kD, it cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids.

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Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
EZ0015-S 1 mL 40 €
EZ0015 5 x 1 mL 134 €

 

We also offer this Product with different specifications or size (Custom Solutions), in higher amounts (Bulk Inquiries) or under OEM Basis (OEM Partnerships).

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Highly stable at high temperatures.
  • Highly active under denaturing conditions.
  • High Quality: free of RNases, DNases and Exonucleases contamination.
  • Molecular Biology Grade: proven performance for general Digestion of Proteins.
  • Risk-free: product covered by our Quality 100% Guarantee.

Specifications

Storage Buffer: 50 mM Tris, pH 8, 3 mM CaCl2, glycerol 50%

Unit Definition:

Unit definition One unit is the amount of enzyme which releases at 37 °C in 1 min as many folin-positive amino acids and peptides from hemoglobin as 1 μmol of tyrosine.

Includes

Includes for 5 mL:

– 5 x 1 mL Proteinase K Solution (20 mg/mL). Storage Buffer: 50 mM Tris, pH 8, 3 mM CaCl2, glycerol 50%

Download documentation

Data Sheet
MSDS

Applications

  • Isolation of genomic DNA from cultured cells and tissues.
  • Removal of DNases and RNases during DNA and/or RNA purification.
  • Determination of enzyme locations.

Quality Control

  • Enzyme activity is assayed by digesting hemoglobin at a concentration of 16.7 mg/mL in a solution of 0.08 M potassium phosphate (pH 7.5), 5 M urea, 4 mM NaCl, 3 mM CaCl2.
  • Free of detectable RNase, DNase and exonuclease activities.

Storage, Shipping & Guarantee​

  • Shipment at Ambient Temperature.
  • Storage: 4 °C or -20 °C.
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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  • slidebg1

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    Proteinase K

    Proteinase K isolated from Tritirachium album is used for protease digestion during DNA and RNA preparation. It is a serine protease that exhibits broad cleavage specificity. With a molecular weight 28.900 kD, it cleaves peptide bonds adjacent to the carboxylic...


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