We  also offer this Product with different specifications or size (Custom Solutions), in higher amounts (Bulk Inquiries) or under OEM Basis (OEM Partnerships).

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

• Optimized: recognition sites for Not I and EcoR I at either side for the insertion of the cloning point.
• Flexible: allows removing the desired insert DNA with other restriction digestion.
• Unprecedented efficiency: more than 2,500 positive colonies expected under optimal conditions.
• Easy-to-use: eliminate screening of recombinants due to its its minumum background (lower than 1%).
• Time-saving protocol: avoids any step required after PCR, just 19 minutes from PCR to plating.
• Powerful: obtain 5-fold more positive colonies using 10-fold less DNA insert.
• High stability: eliminates cloning bias or pitfalls.
• Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers.
• Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert.
• Eliminates positive selection vector.
• Cost avoidance: removes expensive primer phosphorylation use.
• Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation.
• Risk-free: product covered by our Quality 100% Guarantee.

Includes for 20 rxn:

– 20 µL pSpark® Done (20 ng/µL)

– 20 µL T4 DNA Ligase (5U/Weiss)

– 200 µL T4 DNA Ligase Buffer (5x)

– 150 µL PEG 6000 10x

– 5 µL Insert Control 1 kb (20 ng/µL)

• Cloning of High Fidelity PCR amplified products.
• Production of ssDNA.
• Blue/white screening for recombinants.
• In vitro transcription from T7/SP6 dual-opposed promoters.
• One restriction enzyme allows gene fragment excision.

• Functional test using a 1.0 kb PCR fragment.

• Recommendations: All pSpark® DNA cloning vectors are stable for at least 1 month at 4 °C and even at 20 – 25 °C for up to 2 days although storage temperatures above -20 °C are not recommended. In case of incident, such as a power failure, stored vectors should be tested with the supplied control insert before considering discarding it. However, please note that T4 DNA Ligase is extremely temperature-sensitive and storage temperatures above    -20 °C inactivates the enzyme.

• Shipped in: Gel Pack.
• Storage: -20 ºC (NON Frost-Free Freezer).
• Shelf Life: 24 months from the date of purchase, if it is properly stored

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• Oliveras, À., Camó, C., Caravaca-Fuentes, P., Moll, L., Riesco-Llach, G., Gil-Caballero, S., … & Planas, M. (2022). Peptide Conjugates Derived from flg15, Pep13, and PIP1 That Are Active against Plant-Pathogenic Bacteria and Trigger Plant Defense Responses. Applied and Environmental Microbiology, e00574-22.
• Morales de los Ríos Martín, L. (2022). Influencia del transportador de nitrato NRT1. 4/NPF6. 2 en la nutrición de potasio en Arabidopsis thaliana.
• Oliveras, À., Camó, C., Caravaca-Fuentes, P., Moll, L., Riesco-Llach, G., Gil-Caballero, S., … & Planas, M. (2022). Peptide Conjugates Derived from flg15, Pep13, and PIP1 That Are Active against Plant-Pathogenic Bacteria and Trigger Plant Defense Responses. Applied and Environmental Microbiology, e00574-22.
• Sueiro, R., Lamas, J., Palenzuela, O., Gulias, P., Diez-Vives, C., García-Ulloa, A., & Leiro, J. M. (2022). A real-time quantitative PCR assay using hydrolysis probes for monitoring scuticociliate parasites in seawater. Aquaculture, 738303.
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• Moreno Cabezuelo, J. Á. (2019). Glucose uptake in marine cyanobacteria: regulation, expression of the transporter and effects on the proteome and metabolome.
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• Olmos, G., Martínez‐Miguel, P., Alcalde‐Estevez, E., Medrano, D., Sosa, P., Rodríguez‐Mañas, L., … & López‐Ongil, S. (2017). Hyperphosphatemia induces senescence in human endothelial cells by increasing endothelin‐1 production. Aging cell, 16(6), 1300-1312.
• Laguna, J. I. R. (2017). Proliferative and Positional Instructions Underliying Planarian Regeneration and Tissue Renewal(Doctoral dissertation, Universitat de Barcelona).
• Lindo, L., McCormick, S. P., Cardoza, R. E., Kim, H. S., Brown, D. W., Alexander, N. J., … & Gutierrez, S. (2019). Role of Trichoderma arundinaceum tri10 in regulation of terpene biosynthetic genes and in control of metabolic flux. Fungal Genetics and Biology, 122, 31-46.
• Oliveras, À., Baró, A., Montesinos, L., Badosa, E., Montesinos, E., Feliu, L., & Planas, M. (2018). Antimicrobial activity of linear lipopeptides derived from BP100 towards plant pathogens. PloS one, 13(7), e0201571.
• Álvarez-Cao, M. E., Cerdán, M. E., González-Siso, M. I., & Becerra, M. (2019). Bioconversion of Beet Molasses to Alpha-Galactosidase and Ethanol. Frontiers in microbiology, 10.
• Álvarez-Escribano, I., Vioque, A., & Muro-Pastor, A. M. (2018). NsrR1, a nitrogen stress-repressed sRNA, contributes to the regulation of nblA in Nostoc sp. PCC 7120. Frontiers in microbiology, 9, 2267.
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• Burnat, M., Picossi, S., Valladares, A., Herrero, A., & Flores, E. (2019). Catabolic pathway of arginine in Anabaena involves a novel bifunctional enzyme that produces proline from arginine. Molecular Microbiology.
• Camó, C., Bonaterra, A., Badosa, E., Baró, A., Montesinos, L., Montesinos, E., … & Feliu, L. (2018). Antimicrobial peptide KSL-W and analogues: Promising agents to control plant diseases. Peptides.

• Rengel, R., Smith, R. T., Haslam, R. P., Sayanova, O., Vila, M., & León, R. (2018). Overexpression of acetyl-CoA synthetase (ACS) enhances the biosynthesis of neutral lipids and starch in the green microalga Chlamydomonas reinhardtii. Algal Research, 31, 183-193.
• Ruiz-Roldán, C., Pareja-Jaime, Y., González-Reyes, J. A., & G. Roncero, M. I. (2015). The transcription factor Con7-1 is a master regulator of morphogenesis and virulence in Fusarium oxysporum. Molecular Plant-Microbe Interactions, 28(1), 55-68.
• Parejo-Farnés, C., Albaladejo, R. G., Arroyo, J., & Aparicio, A. (2013). A phylogenetic hypothesis for Helianthemum (Cistaceae) in the Iberian Peninsula/Una hipótesis filogenética para el género Helianthemum (Cistaceae) en la Península Ibérica. Botanica complutensis, 37, 83.
• Burnat, M., Schleiff, E., & Flores, E. (2014). Cell envelope components influencing filament length in the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. Journal of bacteriology, 196(23), 4026-4035.
• Rodríguez, M. F. R., Sánchez-García, A., Salas, J. J., Garcés, R., & Martínez-Force, E. (2015). Characterization of soluble acyl-ACP desaturases from Camelina sativa, Macadamia tetraphylla and Dolichandra unguis-cati. Journal of plant physiology, 178, 35-42.
• Rodríguez-Rodríguez, M. F., Salas, J. J., Venegas-Calerón, M., Garcés, R., & Martínez-Force, E. (2016). Molecular cloning and characterization of the genes encoding a microsomal oleate Δ 12 desaturase (CsFAD2) and linoleate Δ 15 desaturase (CsFAD3) from Camelina sativa. Industrial Crops and Products, 89, 405-415.
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• Chamizo-Ampudia, A., Galvan, A., Fernandez, E., & Llamas, A. (2011). The Chlamydomonas reinhardtii molybdenum cofactor enzyme crARC has a Zn-dependent activity and protein partners similar to those of its human homologue. Eukaryotic cell, 10(10), 1270-1282.
• Merino-Puerto, V., Herrero, A., & Flores, E. (2013). Cluster of genes that encode positive and negative elements influencing filament length in a heterocyst-forming cyanobacterium. Journal of bacteriology, 195(17), 3957-3966.
• Merino-Puerto, V., Herrero, A., & Flores, E. (2013). Cluster of genes that encode positive and negative elements influencing filament length in a heterocyst-forming cyanobacterium. Journal of bacteriology, 195(17), 3957-3966.
• Rodríguez-Rodríguez, M. F., Salas, J. J., Garcés, R., & Martínez-Force, E. (2014). Acyl-ACP thioesterases from Camelina sativa: Cloning, enzymatic characterization and implication in seed oil fatty acid composition. Phytochemistry, 107, 7-15.
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• Ruiz-Roldán, C., Pareja-Jaime, Y., González-Reyes, J. A., & G. Roncero, M. I. (2015). The transcription factor Con7-1 is a master regulator of morphogenesis and virulence in Fusarium oxysporum. Molecular Plant-Microbe Interactions, 28(1), 55-68.
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• Domínguez Martín, M. A. (2015). Diversity of regulatory mechanisms in the C/N metabolism of the marine cyanobacteria Prochlorococcus and synechococcus.
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This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.