pSpark® V DNA Cloning Kit

pSpark® V DNA Cloning Kit

For highly Efficient, Accurate & Easy Cloning with pBR322 and Transcription-free conditions

Product Overview:

pSpark® V is a highly efficient, accurate and easy-to-use DNA cloning system developed with low copy number, as a help for cloning of inserts with the highers kb. This low copy variant is also transcription-free, for the most demanding cloning tasks. In this vector, the lac promoter has been eliminated and therefore blue/white screening is not allowed (alpha-peptide coding region has been truncated).

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Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
C0005-S 10 rxn 89 €
C0005 20 rxn 169 €

Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions.
  • Transcription-free.
  • Easy-to-use: eliminate screening of recombinants due to its minumum background (lower than 1%).
  • High stability: removes cloning bias or pitfalls.
  • Time-saving protocol: avoids any step required after PCR, just 19 minutes from PCR to plating.
  • Powerful: obtain 5-fold more positive colonies using 10-fold less DNA insert.
  • Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers.
  • Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert.
  • Optimized: truncated alpha-peptide coding region.
  • Cost avoidance: avoids expensive primer phosphorylation use.
  • Eliminates positive selection vector.
  • Risk-free: product covered by our Quality 100% Guarantee.


Includes for 20 rxn:

– 20 µL pSpark® V (20 ng/µL)

– 20 µL T4 DNA Ligase (5U/Weiss)

– 200 µL T4 DNA Ligase Buffer (5x)

– 150 µL PEG 6000 (10x)

– 5 µL Insert Control 1 kb (20 ng/µL)

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White Paper


  • Cloning of toxic genes.
  • Cloning of unstable genes, for example genes with repeated sequences.
  • Cloning of high fidelity PCR amplified products.
  • Production of ssDNA.
  • Blue/white screening for recombinants.
  • In vitro transcription from T7/SP6 dual-opposed promoters.

Tables, Figures & Protocols

pSpark V

Quality Control

  • Functional test using a 1.0 kb PCR fragment.


  • Recommendations: All pSpark® DNA cloning vectors are stable for at least 1 month at 4 °C and even at 20 – 25 °C for up to 2 days although storage temperatures above -20 °C are not recommended. In case of incident, such as a power failure, stored vectors should be tested with the supplied control insert before considering discarding it. However, please note that T4 DNA Ligase is extremely temperature-sensitive and storage temperatures above    -20 °C inactivates the enzyme.

Storage, Shipping & Guarantee​

  • Shipped in: Gel Pack.
  • Storage: -20º C (NON Frost-Free Freezer).
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.


  • Calatrava, V., Hom, E. F., Llamas, Á., Fernández, E., & Galván, A. (2018). Ok, thanks! A new mutualism between Chlamydomonas and Methylobacteria facilitates growth on amino acids and peptides. FEMS Microbiology Letters.
  • Velázquez-Palmero, D., Romero-Segura, C., García-Rodríguez, R., Hernández, M. L., Vaistij, F. E., Graham, I. A., … & Martínez-Rivas, J. M. (2017). An Oleuropein β-Glucosidase from Olive Fruit Is Involved in Determining the Phenolic Composition of Virgin Olive Oil. Frontiers in plant science8.
  • Bornikoel, J., Carrión, A., Fan, Q., Flores, E., Forchhammer, K., Mariscal, V., … & Maldener, I. (2017). Role of Two Cell Wall Amidases in Septal Junction and Nanopore Formation in the Multicellular Cyanobacterium Anabaena sp. PCC 7120. Frontiers in cellular and infection microbiology7, 386.
  • Prieto-Dapena, P., Almoguera, C., Personat, J. M., Merchan, F., & Jordano, J. (2017). Seed-specific transcription factor HSFA9 links late embryogenesis and early photomorphogenesis. Journal of experimental botany68(5), 1097-1108.
  • Badosa, E., Montesinos, L., Camó, C., Ruz, L., Cabrefiga, J., Francés, J., … & Feliu, L. (2017). CONTROL OF FIRE BLIGHT INFECTIONS WITH SYNTHETIC PEPTIDES THAT ELICIT PLANT DEFENSE RESPONSES. Journal of Plant Pathology99, 65-73.
  • De Felipe, A. P., Lamas, J., Sueiro, R. A., Folgueira, I., & Leiro, J. M. (2017). New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species. Parasitology144(10), 1394-1411.
  • Suarez‐Bregua, P., Chien, C. J., Megias, M., Du, S., & Rotllant, J. (2017). Promoter architecture and transcriptional regulation of musculoskeletal embryonic nuclear protein 1b (mustn1b) gene in zebrafish. Developmental Dynamics246(12), 992-1000.
  • Ruiz, C., Nadal, A., Foix, L., Montesinos, L., Montesinos, E., & Pla, M. (2018). Diversity of plant defense elicitor peptides within the Rosaceae. BMC genetics19(1), 11.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

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