qMAXSen™ One-Step Green RT-qPCR Kit (High Rox™)
For Greater Specificity, Sensitivity & Yields in cDNA Synthesis and qPCR in a single tube
Product Overview:
qMAXSen™ One-Step Green RT-qPCR Kit (High Rox™) offers greater specificity, sensitivity, and yields in cDNA Synthesis and qPCR in a single tube. The kit includes a qPCR MasterMix supplied in a 4x concentration to perform Real-Time PCR using a proprietary green dye. The qPCR MasterMix contains all the reagent (except PCR Primers and Template) needed for running PCR reactions. In addition, a separate RT mix that comprises a balanced mixture of both RTase and RNase Inhibitor is also supplied.
Available with the option of ROX™ as the internal passive reference dye. The ROX™ dye provides an internal reference to which the reporter-dye signal can be normalized during data analysis.
RT-PCR is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the Reverse Transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR step synthesis, Taq DNA polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. During subsequent rounds of cycling the DNA polymerase exponentially amplifies the double-stranded DNA template.
Quote requestAvailability: In stock! Ready-to-ship immediately
Catalog Numbers, Sizes & Prices
| Cat. No. | Size | Price |
| E0832 | 100 rxn | 144 € |
| E0834 | 500 rxn | 488 € |
We also offer this Product with different specifications or size (Custom Solutions), in higher amounts (Bulk Inquiries) or under OEM Basis (OEM Partnerships).
Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.
Advantages & Features
- Enhanced specificity, sensitivity, and yields in cDNA Synthesis.
- Versatile: compatible with most used real-time PCR instruments.
- Convenient: includes internal passive reference dye.
- Easy: reaction assembly.
Download documentation
Data SheetMSDSApplications
- Detection and Quantification of DNA and cDNA targets.
- Gene Expression.
- Standard and fast qPCR platforms.
- High Throughput Applications.
Quality Control
- Functionally tested in RT qPCR based on specific probe for activity, processivity, efficiency, sensitivity and heat.
Storage, Shipping & Guarantee
- Shipment at Gel Pack.
- Storage: Upon receipt of the kit, immediately store the components at -20 °C in a freezer without a defrost cycle. It is recommended to avoid repeated freezing and thawing.
- Shelf Life: 24 months from the date of purchase, if it is properly stored.
Citations
Ruiz, Leticia, et al. «Resistance to Cucumber Green Mottle Mosaic Virus in Cucumis melo.» Plants 10.6 (2021): 1077.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
