qMAXSen™ One-Step Probe RT-qPCR Kit (High Rox™)

qMAXSen™ One-Step Probe RT-qPCR Kit (High Rox™)

For Greater Specificity, Sensitivity & Yields in cDNA Synthesis and qPCR in a single tube

Product Overview:

qMAXSen™ One-Step Probe RT-qPCR Kit (High Rox™) offers greater specificity, sensitivity, and yields in cDNA Synthesis and qPCR in a single tube. The kit includes a qPCR MasterMix supplied in a 2x concentration to perform Real-Time PCR. The qPCR MasterMix contains all the reagent (except PCR Primers, Probe and Template) needed for running PCR reactions. The Mix is compatible with many probe technologies. In addition, a separate RT mix that comprises a balanced mixture of both RTase and RNase Inhibitor is also supplied.

Available with the option of ROX™ as the internal passive reference dye. The ROX™ dye provides an internal reference to which the reporter-dye signal can be normalized during data analysis.

 

RT-PCR is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the Reverse Transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR step synthesis, Taq DNA polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. During subsequent rounds of cycling the DNA polymerase exponentially amplifies the double-stranded DNA template.

Quote request

Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
E0842 100 rxn 179 €
E0854 500 rxn 466 €

Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Enhanced specificity, sensitivity, and yields in cDNA Synthesis.
  • Versatile: compatible with most used real-time PCR instruments.
  • Compatible: with a wide range of probe technologies including Taqman®, Molecular Beacons® and Scorpion® Probes.
  • Convenient: includes internal passive reference dye.
  • Easy: reaction assembly.

 

Includes

Includes for 100 rxn:

– 1 mL qMAXSen™ One-Step RT-qPCR (2x)

– 100 μL RT mix

– 1 mL RNase-free Water

Download documentation

Data Sheet

Applications

  • One step qRT-PCR based on Specific Probes.
  • Detection and Quantification of DNA and cDNA targets.
  • Gene Expression.
  • Standard and fast qPCR platforms.
  • High Throughput Applications.

Quality Control

  • Functionally tested in RT qPCR based on specific probe for activity, processivity, efficiency, sensitivity and heat.

Storage, Shipping & Guarantee​

  • Shipment at Gel Pack.
  • Storage: Upon receipt of the kit, immediately store the components at -20 °C in a freezer without a defrost cycle. It is recommended to avoid repeated freezing and thawing.
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Current Promotions


AgaPure™ Agarose Standard: 169€ (500g) Learn more Here


BrightMAX™ DNA Ladders from 34 € (50 µg) Learn more Here


CANFAST™ Transfection Reagent: 99 € (1.5 mL) Learn more Here


Clean-Easy™ Agarose Purification Kit: 59 € (50 rxn) Learn more Here


Clean-Easy™ PCR Purification Kit: 56 € (50 rxn) Learn more Here


CVX5α™ Chemically Competent Cells (40 x 50 µL): 299€ (40 rxn) Learn more Here


DNase I (10 mg/mL): 53 € (1 mL) Learn more Here


FastPANGEA™ High Fidelity DNA Polymerase: 63 € (100 units) Learn more Here


FastPANGEA™ Long PCR DNA Polymerase (5 U/μL): 29 € (100 U) Learn more Here


HigherPurity™ Blood Genomic DNA Extraction Kit: 49 € (50 rxn) Learn more Here


HigherPurity™ DNA Purification SPRI Magnetic Beads: 249€ (25 mL) Learn more Here


Horse-Power™ Green-Taq DNA Polymerase: 29 € (100 rxn) Learn more Here


Horse-Power™ Taq DNA Polymerase (500 U) from 49€ Learn more Here


HotBegan™ Hot Start Taq DNA Polymerase: 63 € (500 U) Learn more Here


HotBegan™ HS Red-Taq MasterMix: 99€ (250 rxn) Learn more Here


IPTG: 65 € (5 gr) Learn more Here


PRImeDETECT™ Campylobacter Detection Kit: 419€ (96 rxn) Learn more Here


PRImeDETECT™ Legionella spp Detection Kit: 279€ (96 rxn) Learn more Here


PRImeDETECT™ Listeria monocytogenes Detection Kit: 279€ (96 rxn) Learn more Here


PRImeDETECT™ Salmonella Detection Kit: 249€ (96 rxn) Learn more Here


PRImeDETECT™ Salmonella Plus Detection Kit: 411€ (96 rxn) Learn more Here


PRImeDETECT™ Legionella spp Plus Detection Kit: 429€ (96 rxn) Learn more Here


PRImeDETECT™ STEC Detection Kit: 429€ (96 rxn) Learn more Here


PRImeZOL™ Reagent: 79 € (100 mL) Learn more Here


Proteinase K (Lyophilized): 292 € (500 mg) Learn more Here


qMAXSen™ qPCR MasterMixes from 499 € (2,000 rxn) Learn more Here


Saliva Sample Collection Kit: 649 € (100 units) Learn more Here


T4 DNA Ligase: 39 € (300 U) Learn more Here


TruePure™ dNTP Mix, 1mL from 29€ Learn more Here


TruePure™ dNTP Set, 100 mM: 149 € (4 x 0.4 mL) Learn more Here


WideUSE™ Plasmid Purification Kit: 99 € (100 rxn) Learn more Here


X-Gal: 79 € (1 gr) Learn more Here

Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

  • slidebg1

    Related products

    qMAXSen™ Probe qPCR Mastermix (Low ROX™)

    qMAXSen™ Probe qPCR Mastermix 2x (Low ROX™), offers exceptional Sensitivity, Performance & signal-to-noise ratio in real-time PCR protocols. It is based on TaqMan® Probe-based real-time PCR analysis of DNA samples. The optimized MasterMix includes all the components required for real-time PCR, except...


    Learn more
  • slidebg1

    Related products

    qMAXSen™ Probe qPCR Mastermix (ROX™)

    qMAXSen™ Probe qPCR Mastermix 2x (ROX™), offers exceptional Sensitivity, Performance & signal-to-noise ratio in real-time PCR protocols. It is based on TaqMan® Probe-based real-time PCR analysis of DNA samples. The optimized MasterMix includes all the components required for real-time PCR, except sample DNA and Primers, to ensure fast...


    Learn more
  • slidebg1

    Related products

    qMAXSen™ One-Step Probe RT-qPCR Kit (Low Rox™)

    qMAXSen™ One-Step Probe RT-qPCR Kit (Low Rox™) offers greater specificity, sensitivity, and yields in cDNA Synthesis and qPCR in a single tube. The kit includes a qPCR MasterMix supplied in a 2x concentration to perform Real-Time PCR. The qPCR MasterMix contains all the reagent...


    Learn more