ROS Detection Assay Kit (DCFDA / H2DCFDA)

ROS Detection Assay Kit (DCFDA / H2DCFDA)

Highly Sensitive & Safe Assay for Reactive Oxygen Species Measurement

Product Overview:

ROS Detection Assay Kit is a highly sensitive and safe assay to measure Reactive Oxygen Species. ROS include a number of reactive molecules and free radicals, derived from molecular oxygen, that damage DNA and RNA and oxidize proteins and lipids (lipid peroxidation). The most common ROS include superoxide anion (O2•-), hydrogen peroxide (H2O2), hydroxyl radical (HO•) and singlet oxygen (1O2), all of which are more reactive than oxygen (O2) itself. The molecules are produced during the electron transport of mitochondrial aerobic respiration or by oxidoreductase enzymes and metal catalysed oxidation.

It uses the cell permeant reagent Dichlorodihydrofluorescein-diacetate (H2DCFDA), a fluorogenic dye that measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, the acetyl groups on H2DCFDA are cleaved by intracellular esterase to yield the non-fluorescent compound which is rapidly oxidized to highly fluorescent 2’,7’-Dichlorodihydrofluorescein by ROS. The fluorescence intensity is proportional to the ROS levels within the cell cytosol.

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Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price Promo Price
CA093 5×96 assays 235 € 205 €

Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Based on unique fluorescent probe H2DCFDA.
  • Fast & Easy Protocol.
  • Safe: non-Radioactive, no special handling needed.
  • High-Throughput.
  • Accurate & Reproducible results.

Includes

Include :

– 5 mg  Fluorescent Substrate (H2DCFDA)

– 50 mL  10X Assay Buffer

– 500 μL  Positive control (H2O2, 8.8M)

– 1 mL  DMSO

Download documentation

Data Sheet

Applications

• Measurement of intracellular levels of ROS.

Tables, Figures & Protocols

ros detection

Quality Control

  • Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.

Storage, Shipping & Guarantee​

  • Shipment Gel Pack.
  • Storage: 4 °C upon receipt.
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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Frequently asked questions

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Customers Review

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