ROS Detection Assay Kit (DCFDA / H2DCFDA)

ROS Detection Assay Kit (DCFDA / H2DCFDA)

Highly Sensitive & Safe Assay for Reactive Oxygen Species Measurement

Product Overview:

ROS Detection Assay Kit is a highly sensitive and safe assay to measure Reactive Oxygen Species. ROS include a number of reactive molecules and free radicals, derived from molecular oxygen, that damage DNA and RNA and oxidize proteins and lipids (lipid peroxidation). The most common ROS include superoxide anion (O2•-), hydrogen peroxide (H2O2), hydroxyl radical (HO•) and singlet oxygen (1O2), all of which are more reactive than oxygen (O2) itself. The molecules are produced during the electron transport of mitochondrial aerobic respiration or by oxidoreductase enzymes and metal catalysed oxidation.

It uses the cell permeant reagent Dichlorodihydrofluorescein-diacetate (H2DCFDA), a fluorogenic dye that measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, the acetyl groups on H2DCFDA are cleaved by intracellular esterase to yield the non-fluorescent compound which is rapidly oxidized to highly fluorescent 2’,7’-Dichlorodihydrofluorescein by ROS. The fluorescence intensity is proportional to the ROS levels within the cell cytosol.

Quote request

Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
CA093 5 x 96 assays 229 €

 

We also offer this Product with different specifications or size (Custom Solutions), in higher amounts (Bulk Inquiries) or under OEM Basis (OEM Partnerships).

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Based on unique fluorescent probe H2DCFDA.
  • Fast & Easy Protocol.
  • Safe: non-Radioactive, no special handling needed.
  • High-Throughput.
  • Accurate & Reproducible results.

Includes

Include :

– 5 mg  Fluorescent Substrate (H2DCFDA)

– 50 mL  10X Assay Buffer

– 500 μL  Positive control (H2O2, 8.8M)

– 1 mL  DMSO

Download documentation

Data Sheet
MSDS

Applications

• Measurement of intracellular levels of ROS.

Tables, Figures & Protocols

ros detection

Quality Control

  • Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.

Storage, Shipping & Guarantee​

  • Shipment Gel Pack.
  • Storage: 4 °C upon receipt.
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Citations

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Current Promotions


AgaPure™ Agarose Standard: 181€ (500g) Learn more Here


TBARS Assay Kit from 229€ (96 assays) Learn more Here


CVX-Mag™ Viral RNA Extraction Kit, CE-IVD: 336 € (100 rxn) Learn more Here


UltraPure Water, DNASE/RNASE – Free from 34€ (1 L) Learn more Here


qMAXSen™ One-Step RT-qPCR Kits from 144 € (100 rxn) Learn more Here


XTT Cell Proliferation Assay Kit from 91 € (500 assays) Learn more Here


Horse-Power™ Taq DNA Polymerase: 39 € (500 U) Learn more Here


CVX™ Viral RNA Extraction Kit, CE-IVD: 336 € (100 rxn) Learn more Here


GreenSafe DNA Gel Stain: 99 € (1 mL) Learn more Here


Horse-Power™ Taq DNA Polymerase (500 U) from 39€ Learn more Here


Stool Sample Collection and Stabilization Kit: 392 € (100 units) Learn more Here


BrightMAX™ DNA Ladders from 39 € (50 µg) Learn more Here


Proteinase K (Lyophilized): 79 € (100 mg) Learn more Here


HigherPurity™ PCR Clean-Up Magnetic Beads: 261€ (25 mL) Learn more Here


TruePure™ dNTP Mix, 1mL from 23€ Learn more Here


qMAXSen™ qPCR MasterMixes from 117 € (250 rxn) Learn more Here


Saliva Sample Collection Kit: 755 € (100 units) Learn more Here


IPTG: 34 € (5 gr) Learn more Here


PRImeZOL™ Reagent: 84 € (100 mL) Learn more Here


Resazurin Cell Viability Assay from 92 € (2,500 assays) Learn more Here

Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

Leave a Reply

You must be logged in to post a comment.

  • slidebg1

    Related products

    TBARS Assay Kit

    Oxidative stress in the cellular environment results in the formation of highly reactive and unstable lipid hydroperoxides. Decomposition of the unstable peroxides derived from polyunsaturated fatty acids results in the formation of malondialdehyde (MDA), which can be quantified colorimetrically following...


    Learn more
  • slidebg1

    Related products

    Catalase Activity Assay Kit

    Catalase Activity Assay Kit is a fast, easy and highly sensitive for measuring Catalase activity in biological samples. In the assay, catalase first reacts with H2O2 to producing water and oxygen. The unconverted H2O2 reacts 1:1 with the fluorogenic to...


    Learn more
  • slidebg1

    Related products

    SOD Assay Kit

    Superoxide dismutases (SOD) catalyse the breakdown of superoxide radicals and provide the first line of defense against oxygen toxicity. SOD Assay Kit, superoxide ions are generated from the conversion of xanthine and O2 to uric acid and H2O2 by Xanthine...


    Learn more