SRB Cytotoxicity Assay

SRB Cytotoxicity Assay

For a Sensitive, Reproducible & Easy-to-use Colorimetric assay for Cytotoxicity Detection

Product Overview:

Sulforhodamine B (SRB) Cytotoxicity Assay is a sensitive, reproducible and easy-to-use assay based on the ability of SRB to bind to protein components of cells that have been fixed to tissue culture plates. SRB is a bright-pink aminoxanthene dye with two sulfonic groups that bind to basic aminoacid residues under mild acidic conditions and dissociate under basic conditions.

As the binding of SRB is stoichiometric, the amount of dye extracted from stained cells is directly proportional to the cell mass. The fixed dye is solubilized and is measured photometrically at OD 540 nm with a reference filter of 690 nm. The OD values correlate with total protein content and therefore with cell number.

Quote request

Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
CA050 1,000 assays 104 €


We  also offer this Product with different specifications or size (Custom Solutions), in higher amounts (Bulk Inquiries) or under OEM Basis (OEM Partnerships).

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Sensitive.
  • Easy-to-use.
  • Fast: avoids time-sensitive measurement.
  • Reproducible.
  • Great linearity.
  • Good signal-to-noise ratio.
  • Stable end-point.
  • Risk-free: product covered by our Quality 100% Guarantee.


Includes for 1,000 assays:

– 0.4 g SRB Dye

– 100 mL Fixative Reagent

– 100 mL Dye Wash Solution (10x)

– 200 mL SRB Solubization Buffer

Download documentation

Data Sheet


  • Detection of cell toxicity, death, viability or proliferation.
  • High throughput screening.


  • Precautions: Some components of this kit are potentially carcinogenic or corrosive; it is advisable to work in a hood and to wear glasses, gloves and a mask.

Storage, Shipping & Guarantee​

  • Shipped in: Ambient Temperature.
  • Storage: Room Temperature.
  • Recommendations: Protect the product from light.
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.


Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

One Response

  • Brian Rigley dice:

    Before to purchase Canvax´s SRB Cytotoxicity Assay we were purchasing another one from a most known brand. Right now, our lab is saving a lot with a product with equivalent quality and performance.

  • Leave a Reply

    You must be logged in to post a comment.

    • slidebg1

      Related products

      XTT Cell Proliferation Assay Kit

      XTT Cell Proliferation Assay Kit is an optimized, accurate and sensitive colorimetric assay that detects the cellular metabolic activities. During the assay, the yellow tetrazolium salt XTT (sodium 2,3,-bis(2methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium) is reduced to a highly colored formazan dye by dehydrogenase enzymes...

      Learn more
    • slidebg1

      Related products

      Resazurin Cell Viability Assay

      Resazurin Cell Viability Assay is a reliable, sensitive and easy-to-use fluorescent assay that detects cellular metabolic activity. Resazurin (7-Hydroxy3H-phenoxazin-3-one 10-oxide) is a blue dye non-fluorescent until it is irreversibly reduced to the pink colored and highly red fluorescent resorufin by...

      Learn more