T7 Biotin16 RNA Labeling Kit

T7 Biotin16 RNA Labeling Kit

For Easy, Scalable & High-Quality RNA Synthesis with Modified Nucleotides

Product Overview:

T7 Biotin16 RNA Labeling Kit is designed to generate randomly biotin-modified RNA probes by in vitro transcription. Such probes are suited for in situ hybridization and Northern blot hybridization experiments.

Biotin-16-UTP (Biotin-16-uridine-5’-triphosphate) is a biologically active analog of UTP (uridine 5′-triphosphate) that is readily incorporated into RNA during an in vitro transcription reaction by RNA Polymerases such as phage T7 RNA Polymerase.

Since T7 RNA polymerase does not significantly discriminate between the natural and the biotinylated nucleotide, the number of biotinylated positions per RNA transcript can be controlled by adjusting the ratio of the biotinylated nucleoside triphosphate (NTP) to the naturally occurring NTP in the transcription reaction. The biotin-labeled RNA can then be detected by a reporter molecule attached to streptavidin, avidin, or ant-biotin antibody.

Quote request

Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
RS026 30 rxn Quote Request

 

We  also offer this Product with different specifications (Custom Solutions), in higher amounts (Bulk Inquiries) or under OEM Basis (OEM Partnerships).

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • High-Quality Kit: High enzymatic purity, specificity, and activity.
  • Scaleable: Increase the reaction size to produce milligram amounts of RNA.
  • Easy and time-saving protocol with minimal handling steps.
  • Convenient: Rnase Inhibitor included.

Includes

Includes:

– 3 x 40 μl T7 RNA Polymerase mix (include RNase Inhibitor)
– 200 μl T7 Reaction Buffer 10x (HEPES-based)
– 100 μl ATP (100 mM)
– 100 μl GTP (100 mM)
– 100 μl CTP (100 mM)
– 100 μl UTP (100 mM)
– 100 μl DTT (100 mM)
– 30 µl Biotin-16-UTP (10mM)
– 10 µl T7 Control template 1 (200 ng/µl)
– 1.2 ml Nuclease-Free Water

Download documentation

Data Sheet

Applications

  • in situ hybridization.
  • Southern blots.
  • Microarray hybridization.
  • Chromosome mapping.

Advice

  • Avoid freeze/thaw cycles.

Storage, Shipping & Guarantee​

This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

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