T7 Fluorescein RNA Labeling Kit

T7 Fluorescein RNA Labeling Kit

Easy, Convenient & High Quality Kit to generate randomly fluorescein-modified RNA Probes by In Vitro Transcription

Product Overview:

T7 Fluorescein RNA Labeling Kit is designed to generate randomly fluorescein-modified RNA probes by in vitro transcription. Such probes are suited for in situ hybridization and Northern blot hybridization experiments.

The fluorescein labeled analog of uridine triphosphate (Fluorescein-12-UTP) is used for labeling RNA probes. Fluorescein-12-UTP is efficiently incorporated into RNA as substitute for its natural counterpart UTP via in vitro transcription with RNA polymerases and optimized reaction buffer. fluorescein-modified RNA probe may be detected directly via fluorescence spectroscopy.

Quote request

Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
RS028 20 rxn Quote Request

 

We  also offer this Product with different specifications (Custom Solutions), in higher amounts (Bulk Inquiries) or under OEM Basis (OEM Partnerships).

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Quality Kit: High enzymatic purity, specificity, and activity.
  • Convenient: Rnase inhibitor included.
  • Easy and time-saving protocol with minimal handling steps.

Includes

Includes:

– 2 x 40 μl T7 RNA Polymerase mix (include RNase inhibitor)
– 200 μl T7 Reaction Buffer 10x  (HEPES-based)
– 100 μl ATP (100 mM)
– 100 μl GTP (100 mM) 100 μl
– 100 μl CTP (100 mM) 100 μl
– 100 μl UTP (100 mM) 100 μl
– 10 μl Fluorescein-12-UTP (5mM)
– 100 μl DTT (100 mM)
– 10 µl Control template 1 (200 ng/µl)
– 1.2 ml Nuclease-Free Water

Download documentation

Data Sheet

Applications

Fluorescein-labeled RNA is used for hybridization to:

  • in situ hybridization to tissues.
  • Southern blots.
  • plaque or colony lifts.
  • RNase protection experiments.

Advice

  • Avoid freeze/thaw cycles.

Storage, Shipping & Guarantee​

  • Shipped in: Gel Pack.
  • Storage: -20 ºC (NON Frost-Free Freezer).

Safety Statements

This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Current Promotions


AgaPure™ Agarose Standard: 181€ (500g) Learn more Here


BrightMAX™ DNA Ladders from 39 € (50 µg) Learn more Here


CVX™ Viral RNA Extraction Kit, CE-IVD: 336 € (100 rxn) Learn more Here


CVX-Mag™ Viral RNA Extraction Kit, CE-IVD: 336 € (100 rxn) Learn more Here


GreenSafe DNA Gel Stain: 99 € (1 mL) Learn more Here


HigherPurity™ PCR Clean-Up Magnetic Beads: 261€ (25 mL) Learn more Here


Horse-Power™ Taq DNA Polymerase: 39 € (500 U) Learn more Here


Horse-Power™ Taq DNA Polymerase (500 U) from 39€ Learn more Here


IPTG: 34 € (5 gr) Learn more Here


PRImeZOL™ Reagent: 84 € (100 mL) Learn more Here


Proteinase K (Lyophilized): 79 € (100 mg) Learn more Here


qMAXSen™ One-Step RT-qPCR Kits from 144 € (100 rxn) Learn more Here


qMAXSen™ qPCR MasterMixes from 117 € (250 rxn) Learn more Here


Resazurin Cell Viability Assay from 92 € (2,500 assays) Learn more Here


Saliva Sample Collection Kit: 755 € (100 units) Learn more Here


Stool Sample Collection and Stabilization Kit: 392 € (100 units) Learn more Here


TruePure™ dNTP Mix, 1mL from 23€ Learn more Here


TBARS Assay Kit from 229€ (96 assays) Learn more Here


UltraPure Water, DNASE/RNASE – Free from 34€ (1 L) Learn more Here


XTT Cell Proliferation Assay Kit from 91 € (500 assays) Learn more Here

Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

Comments are closed.

  • slidebg1

    Related products

    T7 Biotin16 RNA Labeling Kit

    T7 Biotin16 RNA Labeling Kit is designed to generate randomly biotin-modified RNA probes by in vitro transcription. Such probes are suited for in situ hybridization and Northern blot hybridization experiments. Biotin-16-UTP (Biotin-16-uridine-5’-triphosphate) is a biologically active analog of UTP (uridine...


    Learn more
  • slidebg1

    Related products

    T7 Digoxigenin RNA Labeling Kit

    T7 Digoxigenin RNA Labeling Kit is designed to generate randomly Digoxigenin-modified RNA probes by in vitrotranscription with Dioxigenin-11-UTP. Digoxigenin (DIG) is a steroid hapten derived from plants of the genus Digitalis (D. lanata, and the purple foxglove D. purpurea). It...


    Learn more
  • slidebg1

    Related products

    T7 Fluorescein RNA Labeling Kit

    T7 Fluorescein RNA Labeling Kit is designed to generate randomly fluorescein-modified RNA probes by in vitro transcription. Such probes are suited for in situ hybridization and Northern blot hybridization experiments. The fluorescein labeled analog of uridine triphosphate (Fluorescein-12-UTP) is used...


    Learn more
  • slidebg1

    Related products

    T7 RNA Polymerase

    T7 RNA Polymerase is a recombinant protein from the bacteriophage T7 that is routinely used to produce RNA sequences from DNA templates. The protein shows highly stringent specificity for its own promoter sequence that besides its consistent yields makes it suitable...


    Learn more
  • slidebg1

    Related products

    CVX™ T7 ARCA mRNA Synthesis Kit

    CVX™ T7 ARCA mRNA Synthesis Kit is designed for in vitro ARCA-capped (m)RNA Synthesis by T7 RNA Polymerase. The cap structure is added to the mRNA by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA). ARCA is incorporated into mRNA exclusively...


    Learn more