T7 RNA Polymerase
High Quality & Proven Performance in RNA In Vitro Transcription Reactions
Product Overview:
T7 RNA Polymerase is a recombinant protein from the bacteriophage T7 that is routinely used to produce RNA sequences from DNA templates. The protein shows highly stringent specificity for its own promoter sequence that besides its consistent yields makes it suitable for producing large amount of a designed RNA. This recombinant version is purified from E. coli.
GMP-grade is also available. Learn more.
Quote requestAvailability: In stock! Ready-to-ship immediately
Catalog Numbers, Sizes & Prices
| Cat. No. | Size | Concentration | Price |
| EZ0025 | 5,000 U | 50 U/µL | 62 € |
Would you like to test a Free Sample? Please, inquiry at Free Sample Request.
We also offer this Product with different specifications (GMP-grade is also available) or size (Custom Solutions), in higher amounts (Bulk Inquiries) or under OEM Basis (OEM Partnerships).
Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.
Advantages & Features
- Isolated form a recombinant source.
- High-Purity: ≥ 95% determined by SDS.
- Extensivelly quality controlled: Endo/exonucleases, non-specific DNAse and RNAses free, Purity and Promoter Specificity.
- GMP-grade available.
Specifications
Protein information
Predicted MW: 98,855 Daltons
Purity: ≥ 95% as determined by SDS-polyacrylamide gels with Coomassie® blue staining.
Buffer: 1X Reaction Buffer: 40 mM Tris-HCl, 6 mM MgCl2, 1 mM DTT, 2 mM spermidine (pH 7.9 @ 25°C).
Biological activity: One unit is defined as the amount of enzyme that will incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50 μl in 1 hour at 37°C.
Gene Information
Synonyms: T7RNAP
Official Symbol: T7p07
Specie: Escherichia phage T7
GenBank accession number: NC_001604.1
NCBI Protein RefSeq: NP_041960.1
Protein Family: Family of single-subunit RNAPs that includes the phage RNAPs (T3,K11, SP6, N4, and others) as well as the mitochondrial RNAPs.
Includes
Includes:
– 5,000 U T7 RNA Polymerase (50 U/µL)
– T7 RNA Polymerase Reaction Buffer
Download documentation
Data SheetBrochureApplications
- Synthesis of mRNA, RNA standard template for RT-PCR, RNA probes for hybridization.
- Preparation of RNA vaccines.
- Studies of RNA secondary structure and RNA-protein interactions, RNA splicing.
Quality Control
Quality Tests for Research Use Only version includes: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
To be informed about our Safety Staments for GMP Products, please contact us.
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