TBARS Assay Kit

TBARS Assay Kit

Accurate, Simple & Fast Colorimetric or Fluorometric MDA Quantitation

Product Overview:

Oxidative stress in the cellular environment results in the formation of highly reactive and unstable lipid hydroperoxides. Decomposition of the unstable peroxides derived from polyunsaturated fatty acids results in the formation of malondialdehyde (MDA), which can be quantified colorimetrically following its controlled reaction with thiobarbituric acid. Thiobarbituric Acid Reactive Substances (TBARS) assay was proposed over 40 years ago and is now the most commonly used of method to screening and monitoring lipid oxidation.

TBARS method has been used to evaluate a wide range of samples that include human and animal tissues and fluids, drugs, foods and natural products. The sensitivity of measuring Thiobarbituric Acid Reactive Substances (TBARS) has made this assay the method of choice for screening and monitoring lipid peroxidation, a major indicator of oxidative stress. Even though there remains a controversy cited in literature regarding the specificity of TBARS toward compounds other than MDA, it still remains the most widely employed assay used to determine lipid peroxidation.

This assay is based on the reaction of malondialdehyde (MDA) with thiobarbituric acid (TBA); TBA reacts with MDA to form a pink chromogen, which can be detected spectrophotometrically at 532 nm.

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Catalog Numbers, Sizes & Prices

Cat. No. Size Price
CA995 96 assays 224 €

Different specifications, sizes or Bulk Orders? please visit Custom Solutions or OEM partnerships.

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Accurate.
  • Simple & Fast Protocol.
  • Versatile: Colorimetric or Fluorometric MDA Quantitation.
  • Standardized tool for assaying lipid peroxidation in Plasma, Serum, Urine, Tissue homogenates, and Cell Lysates.



Includes for 96 wells:

– 4 x 0.53 g Thiobarbituric Acid (TBA)
– 40 mL Diluent 1 (contain Acetic Acid)
– 20 mL Diluent 2 (contain NaOH)
– 10 mL SDS Solution
– 250 μL MDA Standard (10mM)
– 20 mL H2O2 solution
– 1 unit 96-Well Solid Black plate
– 1 unit 96-Well Solid Clear plate
– 2 Adhesive strips

Download documentation

Data Sheet


  • For quantitative determination of lipid Peroxides (thiobarbituric acid reactive substances, TBARS).
  • Evaluation of drug effects on lipid Peroxidation.

Tables, Figures & Protocols

tbars principle

Quality Control

  • Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.

Storage, Shipping & Guarantee​

  • Shipment: Gel Pack.
  • Storage: 4 °C upon receipt.
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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Frequently asked questions

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