XTT Cell Proliferation Assay Kit

XTT Cell Proliferation Assay Kit

For an Optimized, Accurate & Sensitive Colorimetric Detection of Cellular Metabolic Activities

Product Overview:

XTT Cell Proliferation Assay Kit is an optimized, accurate and sensitive colorimetric assay that detects the cellular metabolic activities. During the assay, the yellow tetrazolium salt XTT (sodium 2,3,-bis(2methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium) is reduced to a highly colored formazan dye by dehydrogenase enzymes in metabolically active cells.

This conversion only occurs in viable cells and thus, the amount of the formazan produced is proportional to viable cells in the sample. The formazan dye formed in the assay is soluble in aqueous solution and quantified by measuring the absorbance at wavelength 450 nm using a spectrophotometer. An electron coupling reagent, such as PMS (N-Methylphenazonium methyl sulphate), can significantly improve the efficiency of XTT reduction in cells.

Quote request

Availability: In stock! Ready-to-ship immediately

Catalog Numbers, Sizes & Prices

Cat. No. Size Price
CA031-S 500 assays 91 €
CA031 1,000 assays 173 €


We  also offer this Product with different specifications or size (Custom Solutions), in higher amounts (Bulk Inquiries) or under OEM Basis (OEM Partnerships).

Note: Prices may vary between countries, because additional cost may be applied due taxes, shipments or Custom fees.

Advantages & Features

  • Accurate: dye absorbance is proportional to the number of cells in each well.
  • Sensitive: assayed even in low cell concentrations.
  • Fast protocol: results within 2-5 hours with minimal handling steps.
  • Time-saving protocol: avoids solubilisation step.
  • Complete solution: includes all reagents needed for cell washing procedures.
  • Safe: avoids radioactivity.
  • Optimized: for high throughput assays (no washing or other steps that can cause cell loss and variability).
  • Cost avoidance: allows performance directly in a microtiter plate.
  • Risk-free: product covered by our Quality 100% Guarantee.


Includes for 1,000 assays:

– 2 x 25 mL XTT Cell Proliferation Assay Kit Reagent

– 1 mL Activation Reagent

Download documentation

Data Sheet


  • Spectrophotometric quantification of cell proliferation and viability in response to pharmaceutical, chemical, nutrients and environmental compounds.
  • High Throughput Screening.

Storage, Shipping & Guarantee​

  • Shipped at: Gel Pack.
  • Storage: -20 °C.
  • Shelf Life: 24 months from date of purchase,  if stored properly.
  • Recommendations: Protect XTT solution from light.


Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Frequently asked questions

Do you have any question? Please visit our FAQs section GO

Customers Review

One Response

  • Theo Cahill dice:

    Incredible Performance for a really cheap product! I will purchase it again

  • Leave a Reply

    You must be logged in to post a comment.

    • slidebg1

      Related products

      SRB Cytotoxicity Assay

      Sulforhodamine B (SRB) Cytotoxicity Assay is a sensitive, reproducible and easy-to-use assay based on the ability of SRB to bind to protein components of cells that have been fixed to tissue culture plates. SRB is a bright-pink aminoxanthene dye with...

      Learn more
    • slidebg1

      Related products

      Resazurin Cell Viability Assay

      Resazurin Cell Viability Assay is a reliable, sensitive and easy-to-use fluorescent assay that detects cellular metabolic activity. Resazurin (7-Hydroxy3H-phenoxazin-3-one 10-oxide) is a blue dye non-fluorescent until it is irreversibly reduced to the pink colored and highly red fluorescent resorufin by...

      Learn more